FastQCFastQC Report
Sun 2 Jul 2023
SRR652152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR652152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172442676
Total Bases8.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATTGGAAT12566150.7287146251430243No Hit
GAGACGTCGCTGCCGTGATCGTGGTCCTGTAGGCACCATCAATTGGAATT12143920.704229386929718No Hit
TGATGTCGGCTCTTCCTATCATTGTGAACTGTAGGCACCATCAATTGGAA7426580.430669493901846No Hit
CTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATTGG7299260.423286170762045No Hit
TGATGTCGGCTCTTCCTATCATTGTGAAGCTGTAGGCACCATCAATTGGA6692170.38808084838581375No Hit
ACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAATTG4448440.2579663052781668No Hit
TGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAAT3674930.21311023960217365No Hit
TAGACGTCGCTGCCGTGATCGTGGTCCTGTAGGCACCATCAATTGGAATT3338460.1935982482665718No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGACTGTAGGCACCATCAATT3302470.19151117789427022No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTGTAGGCACCATCAATTGGAATTC2565260.1487601595790592No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA2512960.1457272676515412No Hit
TTTCAGCCCTTTGTCGCTAAGATTCGACTGTAGGCACCATCAATTGGAAT2505000.14526566497959006No Hit
AGACGTCGCTGCCGTGATCGTGGTCCTGTAGGCACCATCAATTGGAATTC2483730.14403221160868554No Hit
CTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAATTG2435550.14123823965710205No Hit
TAGCCGGGTTACGGTGCCCAACTGCGCGCTCTGTAGGCACCATCAATTGG2379110.13796526794794114No Hit
CTGTAGGCACCATCAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2376090.13779013728596975RNA PCR Primer, Index 1 (100% over 33bp)
AAGCCGGGTTACGGTGCCCAACTGCGCGCTCTGTAGGCACCATCAATTGG2333870.13534178743549535No Hit
TCGGGAGGGAAGCGGATGGGGGCCGGCTGTAGGCACCATCAATTGGAATT2282220.13234658919350104No Hit
GAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATTGGAATT2209390.12812315670629004No Hit
CGGGAGGGAAGCGGATGGGGGCCGGCTGTAGGCACCATCAATTGGAATTC2160380.12528105281780713No Hit
TCGAGTCTTTGAACGCAAGTTGCGCCCCACTGTAGGCACCATCAATTGGA2092510.12134525214628426No Hit
TGGAGACGTCGCTGCCGTGATCGTGGTCCTGTAGGCACCATCAATTGGAA2008340.11646420982239919No Hit
CCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATTGGAA2001320.11605711801874381No Hit
TGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATTGGA1944520.11276326980683134No Hit
CCCCCTGTTTTTGGTCCCAAGGCTCGCCTGTAGGCACCATCAATTGGAAT1941280.11257538128206732No Hit
GAGACGTCGCTGCCGTGATCGTGGTCTCTGTAGGCACCATCAATTGGAAT1900400.11020473841405708No Hit
TCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATTGGAA1897940.11006208231192144No Hit
AGCGCGCGACCTATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATTGG1882430.10916265298504181No Hit
ACTATGCCTGAGCGGGGTAAAGCCAGAGCTGTAGGCACCATCAATTGGAA1877050.10885066524947687No Hit
TTGTCGGGAGGGAAGCGGATGGGGGCCGGCTGTAGGCACCATCAATTGGA1777690.10308875049004691No Hit
ACGTTATTTTACTTACTCCGTGAATCGGACTGTAGGCACCATCAATTGGA1730210.10033537173825811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)