FastQCFastQC Report
Tue 27 Jun 2023
SRR652153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR652153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149540193
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATTGG13924050.9311242496523995No Hit
ACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAATTG6577040.43981754122786243No Hit
TGATGTCGGCTCTTCCTATCATTGTGAACTGTAGGCACCATCAATTGGAA3581600.23950751487929403No Hit
TGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATTGGA3136340.20973224235440166No Hit
TGATGTCGGCTCTTCCTATCATTGTGAAGCTGTAGGCACCATCAATTGGA2911150.1946734146584925No Hit
GCCGCTCACGCCCGGTCGTACTCATAACCGCACTGTAGGCACCATCAATT2801470.18733893168106316No Hit
CTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAATTG2702960.18075140507542345No Hit
AGCGCGCGACCTATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATTGG2699390.18051267327172701No Hit
TTGTCGGGAGGGAAGCGGATGGGGGCCGGCTGTAGGCACCATCAATTGGA2677510.17904952148884817No Hit
TGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAAT2564250.17147563799118543No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGACTGTAGGCACCATCAATT2454160.1641137376357405No Hit
AAGCCGGGTTACGGTGCCCAACTGCGCGCTCTGTAGGCACCATCAATTGG2167020.14491221099333476No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGCTGTAGGCACCATCAAT2162010.1445771840083154No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATTG2053690.13733364647991328No Hit
CTGTAGGCACCATCAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1765230.11804384925462816RNA PCR Primer, Index 1 (100% over 33bp)
TCGAGTCTTTGAACGCAAGTTGCGCCCCACTGTAGGCACCATCAATTGGA1712530.11451971310482392No Hit
CGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATTGGAAT1659950.11100360155346328No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA1650540.11037433929217946No Hit
AGCGCGCGACCTATACCCGGCCGTCGGGGCTGTAGGCACCATCAATTGGA1621600.10843907363420348No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC1580910.10571806604529393No Hit
TAAGCGCGCGACCTATACCCGGCCGTCGGGGCTGTAGGCACCATCAATTG1549450.10361428382000283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)