FastQCFastQC Report
Mon 3 Jul 2023
SRR6652803.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6652803.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8229993
Total Bases411.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT939191.1411795854504372No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA888331.0793812339816085Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT732400.8899157022369277No Hit
AGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC729740.886683621723615No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA453310.5508024125901444No Hit
AAGAACCTTACCAGGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCT327630.3980926836754296Illumina PCR Primer Index 6 (95% over 21bp)
AGCTCAATTGGTAGAGCAACTGACTGTAGGCACCATCAATATCTCGTATG235380.28600267339231034No Hit
TACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGT218270.26521286227096424No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA205000.24908891174026515No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATATCTCGTATG192240.2335846458192613No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATATCTCGTATGCCGTC170840.20758219356930194No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC167360.20335375740902817TruSeq Adapter, Index 6 (95% over 23bp)
TTGCCTCTTGGGGTTGTCTGTAGGCACCATCAATATCTCGTATGCCGTCT166730.20258826465587515No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC164270.199599197714992No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATATCTCGTATG155040.18838412134736932No Hit
TGAGCCATTAGCTCAGTTGGTCTGTAGGCACCATCAATATCTCGTATGCC146190.17763077076736272No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT135770.1649697636437844Illumina PCR Primer Index 6 (100% over 23bp)
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT135740.16493331160791996Illumina PCR Primer Index 6 (95% over 21bp)
TTACAGGAGGTGTAACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT134140.16298920302848374Illumina PCR Primer Index 6 (95% over 21bp)
TGGAAGGAGTTGAAAACGCTGTAGGCACCATCAATATCTCGTATGCCGTC127250.15461738545828652No Hit
TTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC125870.1529405918085228Illumina PCR Primer Index 6 (95% over 23bp)
AAGGCTGAGCTGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG125320.1522723044843416TruSeq Adapter, Index 6 (96% over 25bp)
TACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTC124450.15121519544427317No Hit
TTTAAGGAGGTGTGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT121240.14731482760677928Illumina PCR Primer Index 6 (95% over 22bp)
TTAGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTC112330.13648857295504382No Hit
AGAAAGGAGGGAAATGCTGTAGGCACCATCAATATCTCGTATGCCGTCTT108740.1321264793299338Illumina PCR Primer Index 6 (95% over 22bp)
AAGAACCTTACCAGGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTT106330.129198165782158TruSeq Adapter, Index 6 (95% over 22bp)
TGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC105190.12781298841930971Illumina PCR Primer Index 6 (96% over 26bp)
ATAAGGAGGTGTCCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC104230.126646523271648Illumina PCR Primer Index 6 (95% over 23bp)
TAAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATATCTCGTAT102970.12511553776534198No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT101080.12281905950588294No Hit
TAACCGGAGGAAGGTGGGGCTGTAGGCACCATCAATATCTCGTATGCCGT95580.11613618626407093No Hit
AGGGCTAGCCTCAAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTT88520.10755780715730864Illumina PCR Primer Index 6 (100% over 21bp)
TGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT83550.1015189198824349Illumina PCR Primer Index 6 (95% over 24bp)
TTGCCGCGGTGAATACGTTCCCGGGCCCTGTAGGCACCATCAATATCTCG83550.1015189198824349No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT83030.10088708459411813No Hit
AAGAACCTTACCAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC82440.10017019455545101RNA PCR Primer, Index 6 (95% over 23bp)
CTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC82350.10006083844785772Illumina PCR Primer Index 6 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)