FastQCFastQC Report
Tue 4 Jul 2023
SRR6656329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6656329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6297815
Total Bases787.2 Mbp
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA119582518.987934704337935Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2338013.712414543774309Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1490732.3670590514329173Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAA902501.433036696060459No Hit
ACCTCCTTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA837011.329048249273756Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC664711.0554612988790557No Hit
TCCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAA485070.7702195126404952No Hit
TACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGC368420.5849965424516281No Hit
ACTGCCAGACATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT364490.578756282933049Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC348920.5540334227029533Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCTGCGGTTGGATCACCTCCTTCTGTAGGCACCATCAATAGATCGGAAG320250.5085096974109274No Hit
TTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC298180.47346579726460686No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT258930.4111425946935564Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTCCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC246120.39080220679711936Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA227880.36183978093989744Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG224580.3565998683670447No Hit
ACTGCCAGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG219370.3483271579111168Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC216150.34321427352184847Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC205970.32704993716074543No Hit
AGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA202200.32106373400933497No Hit
AGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCGG184890.2935780107862806No Hit
AAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA173320.27520655973540026Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGCA171940.273015323568571No Hit
TCCTGCGGTTGGATCACCTCCTTCTGTAGGCACCATCAATAGATCGGAAG166540.2644409211766303No Hit
AAAACGGCGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT143690.22815849624036275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGA139220.2210607964825896No Hit
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC131940.2095012317764177Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC128180.20353090714795527No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC117740.18695372919020325No Hit
TGATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT109130.1732823209319423No Hit
AAGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCG108400.17212318875673546No Hit
TAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG106520.16913802644250425No Hit
AGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCGGAAG104920.16659746277081813No Hit
AAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATC100970.160325446206343No Hit
ACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA100340.15932509926061658No Hit
ACTGCCAGACACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG95190.15114765994237683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGG93700.14878176002311913No Hit
ACTGTTTCGGCAAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC91550.14536787758929087No Hit
ATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAGC89310.14181108844893028No Hit
TACTGCCAGACATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC84220.1337289202683788No Hit
CCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAAG81490.12939408350356432No Hit
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT76910.12212171999336277Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCTCCTTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG75170.1193588570004041Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC75110.11926358586271588No Hit
TACTGTTTCGGCAAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAG74590.1184379026694179No Hit
CACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC74580.11842202414646984No Hit
ACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGA73600.11686592889756209No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCG73570.11681829332871797No Hit
TACTGCCAGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT71880.1141348229504995Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGGAACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCAC69590.11049864119539873No Hit
TACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCA69320.11006992107580169No Hit
TATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAG67470.1071323943304146No Hit
TTGGAGGTTGTGCCCTTGAGGCGCTGTAGGCACCATCAATAGATCGGAAG67000.10638610375185681No Hit
ACGACCTTGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT65950.10471885884231276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAG64870.10300397836392464No Hit
AGCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA64630.10262289381317172Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)