FastQCFastQC Report
Tue 4 Jul 2023
SRR6656330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6656330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5755429
Total Bases719.4 Mbp
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA91287715.861146058790752Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3810876.621348295670053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAA1989823.4572922365995655No Hit
ACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGCA1144071.9878101180641792No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC954021.6576001545671053Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAA790251.3730514267485534No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG780871.3567537710916076No Hit
ACTGCCAGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG712051.2371797132759348Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGCGGTTGGATCACCTCCTTCTGTAGGCACCATCAATAGATCGGAAG650781.1307237045231555No Hit
TACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGC572800.9952342388377999No Hit
ACTGCCAGACATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT304950.5298475578449495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC298170.518067376037477No Hit
AAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGG264580.45970508888216677No Hit
AAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATC264390.4593749657931668No Hit
TCCTGCGGTTGGATCACCTCCTTCTGTAGGCACCATCAATAGATCGGAAG251940.43774321601395827No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT249250.43306936806969554Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAA247730.43042838335769584No Hit
CCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAAG239410.41597246703938145No Hit
AAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCG238660.4146693495828026No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCA212780.3697031098811227No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC188030.3267002338140215Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCG157710.27401953876939494No Hit
ACTGCCAGACACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG151140.26260422984976445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCCTTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA147530.25633189115876504Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAAT136290.23680250420950377No Hit
ACTGCCAGGCATCTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCT117420.20401606900198058Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCCAGGCATCTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCT113890.19788272950634955Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTGCGGTTGGATCACCTCCTTAACTGTAGGCACCATCAATAGATCGGA101560.1764594785201937No Hit
ATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT98540.17121225889503633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC97220.1689187721714576No Hit
ACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGA94790.16469667161214221No Hit
ACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC90750.15767721224603762No Hit
ACTGCCAGGCATCTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCT89660.15578334820914305Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGG87510.1520477448336171No Hit
ATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGA86900.15098787596893298No Hit
TAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCG85880.14921563622798578No Hit
TAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATC85030.14773876977719644No Hit
TACTGCCAGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT82030.14252629995088115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA82000.142474175252618No Hit
ACTGCCAGGCATCTGTAGGCACATCAATAGATCGGAAGAGCACACGTCTG81220.14111893309777604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTGCGGTTGGATCACCTCCTTCTGTAGGCACCATCAATAGATCGGAAGA80630.14009381403193402No Hit
TAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT78550.1364798349523554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCG77550.13474234501025031No Hit
ACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAG76450.13283110607393472No Hit
AAAACCATGCACCGAAGCTGCGGCAGCACTGTAGGCACCATCAATAGATC74240.12899125330188244No Hit
TAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGA73750.12813988323025094No Hit
TTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC73720.1280877585319878No Hit
ATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAGC73170.12713213906382997No Hit
AAGCGGGTGAAAAGCCCGCTCGCCGGCTGTAGGCACCATCAATAGATCGG70670.12278841420856725No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCG70210.12198916883519889No Hit
TGATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT66390.11535195725635743No Hit
AGCCCGCTCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT64840.11265884784609453No Hit
ACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAA62520.1086278711804107No Hit
TAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGG62070.10784600070646341No Hit
AAGCCCGCTCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG62040.10779387600820026No Hit
ATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAG60290.10475326860951634No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATAGATCGGA60160.10452739491704267No Hit
ACCTGCGGTTGGATCACCTCCTTACTGTAGGCACATCAATAGATCGGAAG60140.10449264511820057No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG58580.10178216080851661No Hit
ACTGCCAGGCATCTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCT57950.1006875421449904Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)