FastQCFastQC Report
Tue 4 Jul 2023
SRR6656331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6656331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5050528
Total Bases631.3 Mbp
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3388766.709714311058171Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2781235.506810377053647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG838321.6598660575686344No Hit
ACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGA758161.5011499787744964No Hit
ACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAG727751.4409384523756725No Hit
TACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC645641.2783613911258387No Hit
ACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAA641551.2702632279238923No Hit
ACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC413470.8186668799776973No Hit
ATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGA394310.7807302523617333No Hit
AAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATC391830.7758198746744895No Hit
ACTGCCAGACATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT390970.7741170824119775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAA386260.7647913247882202No Hit
TACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGC369770.7321412731500548No Hit
AAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCG362060.7168755425175348No Hit
AAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGG334280.6618713924563927No Hit
ACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA332950.6592380044225079No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAAT309330.6124706169335167No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC296720.5875029303866843Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAGC274570.5436461296719868No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCA273920.5423591355200882No Hit
ATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAG273330.5411909408283648No Hit
ACTGCCAGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG268900.5324195806854254Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC261360.5174904485234019No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCG250660.49630454479214847No Hit
TAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCG227190.4498341559535953No Hit
TATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAG217310.430271844844737No Hit
TATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGA209710.41522391322253827No Hit
AAAACGGCGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT193430.38298965969498633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA182740.36182355587376214Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGCA179540.35548758466441527No Hit
TAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGG175000.34649842551115445No Hit
TAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGA168970.33455907976354154No Hit
TATGGTTAAGTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC162190.321134740763738No Hit
TGATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT155210.3073144035633502No Hit
TATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAG152740.30242382578613564No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA149340.29569185637620465No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATAGATCGGA143920.28496030514037346No Hit
AAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA143660.284445507479614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCA140330.27785213743988746No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCG138580.2743871531847759No Hit
TGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC138560.2743475533647175No Hit
ACCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAA137740.2727239607423224No Hit
TTAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGG121650.24086590550532536No Hit
TAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCG120630.2388463146823461No Hit
TAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATC118480.23458933402606622No Hit
ACTGCCAGACACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG111860.22148179358672995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGG108040.21391822795557217No Hit
TGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG107150.21215603596297258No Hit
AGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCGG102660.20326587635985782No Hit
ACCTCCTTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA102130.20221648112830976Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC101020.2000186911150676Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCG99510.19702890470065706No Hit
TACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC96680.19142553016239094No Hit
TACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA94820.18774274689695808No Hit
ACTGTTTCGGCAAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC92740.18362436561088266No Hit
TTAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATC92160.18247597082918854No Hit
AAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATAGATCGGA91520.18120877658731918No Hit
ACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATAGATCGGAAGA90470.17912978603425225No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATA90190.1785753885534344No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGC87720.17368481077621983No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC87270.17279381482490544Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCG84670.16764583821731113No Hit
ATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATAGATCGGAAGAGCA84240.16679444208605518No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC82540.16342845738108966Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCA79620.1576468836525607No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCTGTAGGCACCATCAATAGA78970.15635988950066212No Hit
TACTGCCAGACATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC77800.15404330002724467No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG77770.15398390029715706No Hit
TCCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAA76880.15222170830455747No Hit
TACTGTTTCGGCAAGGGGGTCCTGTAGGCACCATCAATAGATCGGAAGAG76540.15154851136356437No Hit
AAAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCG73560.14564813817486014No Hit
AAAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATC72420.14339094843153033No Hit
TGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGAG71800.14216335400971938No Hit
TACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAA70730.14004476363659404No Hit
AGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA69600.13780737380329344No Hit
TACTGCCAGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT69490.13758957479297215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC69250.13711437695227113No Hit
ACCGAAGCTGCGGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG68910.13644118001127803No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC68460.13555018405996364No Hit
CTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG68420.1354709844198468Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGAT68180.13499578657914577No Hit
TTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC66780.13222379917505656No Hit
AAAATCCTCTCGTGCCGACCACTGTAGGCACCATCAATAGATCGGAAGAG66020.13071900601283667No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATC63650.12602642733591418No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAA62900.12454143408372352No Hit
AAGTCAGATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA61210.12119524928878724Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT60380.11955185675636289No Hit
ACCGAAGCTGCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT60360.11951225693630449No Hit
TACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGG60190.11917565846580794No Hit
TGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAGCA58700.11622547187145581No Hit
AGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCGGAAG57220.11329508518713291No Hit
TCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC57120.11309708608684083No Hit
AAAAGTCATGGTTAAGTGGCTGTAGGCACCATCAATAGATCGGAAGAGCA56550.11196849121517592No Hit
AAGTCCCGTCCTCGGTACCACTGTAGGCACCATCAATAGATCGGAAGAGC55860.1106022974231605No Hit
AAAACGGCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG55540.10996870030222583Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCACCGAAGCTGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA54920.10874110588041487No Hit
TGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAGC54720.10834510767983072No Hit
CACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC54070.10705811352793212No Hit
ACCGAAGCTGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC53560.10604831811644247No Hit
TCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAA52530.10400892738343397No Hit
TTGGAGGTTGTGCCCTTGAGGCGCTGTAGGCACCATCAATAGATCGGAAG52080.10311793143211957No Hit
AGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATAGATCGGAAGA51540.1020487362905423No Hit
TCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA51520.10200913647048387No Hit
TACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGA51360.10169233791001654No Hit
TCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGA50980.1009399413289066No Hit
TCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAG50700.10038554384808876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)