FastQCFastQC Report
Tue 4 Jul 2023
SRR6656332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6656332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5181903
Total Bases647.7 Mbp
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA114959822.184861430250624Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG1663373.2099597387291885No Hit
ACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAG692201.3358026964225305No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC633401.2223308695666437Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAAT628071.212045073016612No Hit
ACCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAA596291.1507162523111685No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCA578561.1165010228867658No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAA501750.9682736245738294No Hit
ACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGCA426790.8236163432623113No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC413870.798683418041596Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATAGATCGGA407090.7855994216796416No Hit
ACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGA373510.7207969736214669No Hit
TACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGC311440.6010147237414517No Hit
ATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCGGAAGA269260.5196160561091167No Hit
TCCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAA254790.4916919517791051No Hit
ACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAA238140.4595608987663412No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCG233200.45002772147606773No Hit
AAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGG227020.43810160089835726No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC224820.43385605635613017No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG221280.42702458922909203No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGC208110.4016092157649419No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT194620.37557630854919516No Hit
AAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATC191110.3688027352113693No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC161530.31171945904815274Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAGC159610.3080142565385728No Hit
ATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGGAAGAG153740.29668637178272156No Hit
AAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCG151940.2932127444299903No Hit
ACCTGCGGTTGGATCACCTCCTTCTGTAGGCACCATCAATAGATCGGAAG148810.28717249242218545No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATC147850.2853198911673955No Hit
CCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATAGATCGGAAG144790.27941472466775236No Hit
TAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGA141340.27275693890835084No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAA131090.25297656092752024No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCG120700.23292601193036613No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA111590.21534559793959865No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCA111210.2146122766095776No Hit
AGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCGG103900.20050548997154133No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCCTGTAGGCACC100110.19319157460106837No Hit
CGCAGGTTCGAATCCTGCACGACCCACCACTGTAGGCACCATCAATAGAT98860.1907793333838939No Hit
TAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATCG97910.18894603005884134No Hit
TGGAGGTTGTGCCCTTGAGGCGCTGTAGGCACCATCAATAGATCGGAAGA96240.18572327579269624No Hit
TAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGG93920.18124615609362044No Hit
ACTGCCAGGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT93900.18120756023414564Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATAGATCG93430.18030055753648805No Hit
TATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAAGAG85030.16409029655707563No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA82360.158937749317191No Hit
TTGGAGGTTGTGCCCTTGAGGCGCTGTAGGCACCATCAATAGATCGGAAG79450.15332205176360886No Hit
ACGTGCCGGTTCGAGTCCGGCCTTCGGCACCACTGTAGGCACCATCAATA77880.15029227679483773No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCA75360.145429198501014No Hit
TAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGCACCATCAATAGATC73440.14172399599143404No Hit
TCCTGCGGTTGGATCACCTCCTTCTGTAGGCACCATCAATAGATCGGAAG72060.13906088168767342No Hit
TAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCGG68300.13180486010641265No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCACTGTAGGCACCATCA67540.13033821744637056No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACATCAATAGATCGGA66430.12819614724551967No Hit
TAAACCATGCACCGAAGCTGCGGCAGCCTGTAGGCACCATCAATAGATCG65900.12717335696943766No Hit
AACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAATAGATCGGAA65290.12599618325545653No Hit
AGCTGCATCCGGGGTTCGAATCCCCGCCTCACCGCCACTGTAGGCACCAT62030.11970505816106553No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCTGTAGGCACCATCAATAGA60420.11659809147334484No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA59170.11418585025617038No Hit
TTGCATCTGATACTGGCAAGCTTCTGTAGGCACCATCAATAGATCGGAAG58200.11231395107164299No Hit
ACCATGCACCGAAGCTGCGGCAGCACTGTAGGCACCATCAATAGATCGGA56860.10972802848683197No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATAGATCGG56650.10932277196234665No Hit
AGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATAGATCGGAAGA56210.10847366305390124No Hit
ACGATGTCGACTTGGAGGTTGTCTGTAGGCACCATCAATAGATCGGAAGA54700.10555967566355448No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC52890.10206675038108587Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)