FastQCFastQC Report
Tue 26 Sept 2023
SRR6826879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6826879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26312716
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length35-82
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAGCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA3380331.2846754398139668Illumina Paired End PCR Primer 2 (100% over 22bp)
AGCTACCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA1047460.3980812927103382Illumina Paired End PCR Primer 2 (100% over 22bp)
AGCTAGGATTCTCTCTTTGCCTTTTGGCTTAGATCAAGTGTAGTATCTGT397750.15116265458875472No Hit
AGCTAGCATTCTCTCTTTGCCTTTTGGCTTAGATCAAGTGTAGTATCTGT394270.1498401001249738No Hit
AGCTAGGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA379870.14436746096450095Illumina Paired End PCR Primer 2 (100% over 22bp)
AGCTAGCTTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC377070.14330333668329792RNA PCR Primer, Index 1 (95% over 22bp)
AGCTAGGATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC308960.11741851354303372RNA PCR Primer, Index 1 (95% over 22bp)
AGCTAGCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC307570.11689025184629363RNA PCR Primer, Index 1 (95% over 22bp)
AGCTAGCTGAACTACCCTGAAATCGGTTTATCCGGAGCTAGGGTATGGTG302480.11495582592082096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)