FastQCFastQC Report
Tue 26 Sept 2023
SRR6826880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6826880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24182774
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length35-82
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGCTCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA2395040.9903909286833678Illumina Paired End PCR Primer 2 (100% over 22bp)
GGAGCTGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA535460.22142207506880723Illumina Paired End PCR Primer 2 (100% over 22bp)
GGAGCGCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA444290.18372168552706153Illumina Paired End PCR Primer 2 (100% over 22bp)
GGAGCTAGAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC416970.1724243877067205RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTCTTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC356590.14745620167479545RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC349080.14435068532667097RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTGATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC339210.1402692677027044RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334460.13830505962632741No Hit
GGAGCTCTATATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC332580.13752764674557186RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGATATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC329020.13605552448201352RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGTAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC311510.1288148332362532RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGAATTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC305750.12643297249521498RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGGAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC300340.12419584287559401RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTCTAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC292140.12080499945953263RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTGAATATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC283110.1170709365269675RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTTCAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC281320.11633074022029069RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGGTATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC277040.11456088536410257RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGCAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC276540.11435412661922077RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTCTTAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC275620.11397369052863829RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGACATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC274390.11346506401622906RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTGAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA273540.11311357414993002RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC273500.11309703345033949RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTCAATATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC266790.11032233109402585RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTCTGTATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC266730.11029752004464004RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGGATTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC264460.10935883534287671RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGCGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA264150.10923064492105002RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGATTTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC263390.1089163716288297RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTCAATTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA261570.10816376979745997RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTCAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA261540.10815136427276704RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGAAGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC260490.10771717090851528RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTGAATTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA258840.10703486705040538RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTGATAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC256860.1062161024206735RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGTTATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC255630.10570747590826429RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTCATAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC252290.10432632749245394RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGTATTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC244950.10129110911758923RNA PCR Primer, Index 1 (95% over 22bp)
GGAGCTAGAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA244090.10093548407639255RNA PCR Primer, Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)