FastQCFastQC Report
Tue 26 Sept 2023
SRR6826885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6826885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27144479
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length35-82
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA2827231.0415488173488243Illumina Paired End PCR Primer 2 (100% over 22bp)
ACTGTGCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA1459860.537811022270864Illumina Paired End PCR Primer 2 (100% over 22bp)
ACTGTCCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC385000.14183363033049926RNA PCR Primer, Index 1 (95% over 22bp)
ACTGTCCTTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC382740.14100104850050724RNA PCR Primer, Index 1 (95% over 22bp)
ACTGTCAGAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC314570.11588728595601339RNA PCR Primer, Index 1 (95% over 22bp)
ACTGTCCTATATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC310810.11450210556629213RNA PCR Primer, Index 1 (95% over 22bp)
ACTGTCCTAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC286700.10562000471624451RNA PCR Primer, Index 1 (95% over 22bp)
ACTGTCGATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC286180.10542843721553838RNA PCR Primer, Index 1 (95% over 22bp)
ACTGTCCTTAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC279750.10305963138949913RNA PCR Primer, Index 1 (95% over 22bp)
ACTGTCCAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA275080.10133920787354217RNA PCR Primer, Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)