FastQCFastQC Report
Tue 26 Sept 2023
SRR6826887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6826887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20990138
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length35-82
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA3361201.6013234405605148Illumina Paired End PCR Primer 2 (100% over 22bp)
ATCTGTCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA550090.26207069243660996Illumina Paired End PCR Primer 2 (100% over 22bp)
ATCTGGCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA528940.2519945319082704Illumina Paired End PCR Primer 2 (100% over 22bp)
ATCTGACTTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC469770.22380510313938862RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAGATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC433440.2064969749126947RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC415290.19785005701248842RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCA322720.15374839365038953Illumina Paired End PCR Primer 2 (100% over 22bp)
ATCTGAGAAGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA300630.1432244037652349RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAGAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA295290.14068035188715766RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAAGAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC286190.1363449825818201RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAGAATTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA285830.1361734734664441RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAAGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGC283430.13503007936393746RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACTATATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC276080.13152843492501098RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA264050.12579717198619658RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAAGAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA255640.12179052848532963RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACAAGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA255020.12149515167551542RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACTTAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC252660.12037081414138391RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACAATTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA250180.11918930690212708RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAAGCGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA245240.11683582070780096RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAAGAATTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC241820.11520648411172904RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAAGTAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC228700.10895592968469286RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACACGTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA226180.10775536587706092RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACTAAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC226070.10770296031402937RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACTTTTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCA224810.1071026784102134RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAGAATATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC222350.10593069945514413RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACTCAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC219960.1047920694947313RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGACTGTATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC216080.10294358236234559RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAAGCAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC213800.1018573579649643RNA PCR Primer, Index 1 (95% over 22bp)
ATCTGAGATAATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTC211630.10082353913061458RNA PCR Primer, Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)