FastQCFastQC Report
Mon 10 Jul 2023
SRR6838651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6838651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25380602
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG957835737.73888814772794TruSeq Adapter, Index 6 (100% over 49bp)
GGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA2191350.8633955963692272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG2014310.7936415377381514No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1925900.7588078486081615TruSeq Adapter, Index 6 (100% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT1878980.7403212894635044No Hit
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG1400290.5517166220092021Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC1235220.48667876356912265Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1225340.4827860269035384TruSeq Adapter, Index 6 (100% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC946740.37301715696105237No Hit
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC817850.32223427954939765Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT662140.2608842768977662No Hit
CGCCGCTGGTGTAGTGGTATCAGATCGGAAGAGCACACGTCTGAACTCCA638640.2516252372579657Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGTCTGAACTCC626230.2467356763247775Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTCGGGGCGGCGGCGGCGGTAGATCGGAAGAGCACACGTCTGAACTCC611510.24093597149508114Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG597560.23543964796422087TruSeq Adapter, Index 6 (100% over 49bp)
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC548090.2159483845182238TruSeq Adapter, Index 6 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACTATCTCGTATG539700.2126427103659716TruSeq Adapter, Index 6 (97% over 49bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG513640.20237502640796304No Hit
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC505970.1993530334702069TruSeq Adapter, Index 6 (100% over 35bp)
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGT503400.19834044913513085No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC501900.1977494466049308Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA492020.1938567099393466Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC465430.18338020508733402Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC464580.183045303653554Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG440460.1735419829679375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT428340.1687666825239212Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCTGGTGTAGTGGTATCAGATCGGAAGAGCACACGTCTGAACTCCAG426500.16804171942020918Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA416890.16425536321006098TruSeq Adapter, Index 6 (100% over 47bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG408480.1609418090240728TruSeq Adapter, Index 6 (100% over 50bp)
ATCCGGCTCGAAGGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC405210.15965342350823672Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCCACGTTGGGCGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC400220.1576873550911046Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAGCTCCAGTCACGCCAATATCTCGTATG382330.15063866491425223TruSeq Adapter, Index 6 (97% over 49bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG378820.14925571899358417No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTGA377530.14874745681761212Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC373980.14734875082947205TruSeq Adapter, Index 6 (98% over 50bp)
CGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT373660.14722267028969604Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG370400.14593822479072796TruSeq Adapter, Index 6 (97% over 49bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG363560.14324325325301582TruSeq Adapter, Index 6 (97% over 49bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG356520.14046948137794368No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC355970.14025278045020365No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAACATCTCGTATG355290.13998485930317964TruSeq Adapter, Index 6 (97% over 49bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG337740.13307012969983928TruSeq Adapter, Index 6 (100% over 49bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG328950.12960685487286708TruSeq Adapter, Index 6 (97% over 49bp)
GCCCCACGTTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC327350.12897645217398704TruSeq Adapter, Index 6 (100% over 35bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT324580.127885067501551Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCAATATCTCGTATG320800.1263957411254469TruSeq Adapter, Index 6 (97% over 49bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC316740.12479609427703882No Hit
CGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGTCTGAACTC303950.11975681270286653Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACACG302820.11931159079678252No Hit
AACCATCCTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC302690.11926037057749851TruSeq Adapter, Index 6 (100% over 36bp)
CCGCGAGGGGGGCCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT298530.11762132356041043Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCCGGCTCGAAGGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC297870.1173612824471224Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGTCGGGGCGGCGGCGGCGGTAGATCGGAAGAGCACACGTCTGAACTC297190.1170933613000984Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCAGATCGGAAGAGCACACGTCTGA295030.11624231765661035Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTGTGAACTCCAGTCACGCCAATATCTCGTATG278710.109812210128034TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTATGAACTCCAGTCACGCCAATATCTCGTATG267090.10523391052741773TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTGAACTCCAG256770.10116781311964153Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)