FastQCFastQC Report
Tue 4 Jul 2023
SRR6841609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6841609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31617013
Total Bases871 Mbp
Sequences flagged as poor quality0
Sequence length15-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN31001919.805451893890165No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT6729732.1285154293354656No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAG5779451.82795572750658No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG3836721.2134985680019805No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC2699120.8536922826960283No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGC2533770.8013944897324741No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAG2255090.7132520709657171No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGC2007970.635091619818735No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAG1948570.6163042663138355No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGG1814180.5737986697225319No Hit
GTCGCGGCGTGGGAAATGTGGCGTACG1675020.5297843917134107No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1491840.4718472298442614No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGA1354470.4283991027235874No Hit
GCACCACCGGCCCGTCTCGC1244570.3936393358853982No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1161780.36745406658117896No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1121630.3547552072676821No Hit
GACGTACGCAGTTTTATCCGGTAAAGCG1003100.31726589731926924No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAA986460.3120029080545971No Hit
GATCACCTGATACCATTCCGAACTCAGA886170.28028264403092096No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGG835870.26437348778013914No Hit
GACGTACGCAGTTTTATCCGGTAAAGC831570.26301346050621544No Hit
GGAATGCCTAAAGGAAAGATCCAAAAAGATA794860.25140262301185756No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGA734390.23227684411553992No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT620350.1962076556694334No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGC607130.1920263625156494No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAG574030.18155731536056235No Hit
CGACTGCATAATTTGTGGTAGTGGGG498790.15776000092102313No Hit
GCGACCGGCTCCGGGACGGCCGGG494380.15636518225172No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG492870.155887591278784No Hit
GCGACCGGCTCCGGGACGGC491530.15546376882597984No Hit
GGAATGCCTAAAGGAAAGATCCAAAAAGA486750.15395192455403678No Hit
GGAATGCCTAAAGGAAAGATCCAAAAAGAT463610.14663308010785206No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT449650.14221773574878818No Hit
TCGACTGCATAATTTGTGGTAGTGGGG448420.14182870469136347No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAA443970.14042123460555872No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAG441680.1396969410108412No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAAC440660.13937432989005No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAG429060.13570541910458145No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGC424910.13439283464253882No Hit
GGAATGCCTAAAGGAAAGATCCAAAAAG416600.13176450286432814No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGA415700.13147984599304177No Hit
AACTGGTTATATATTGACACTAAGGGACG383710.12136187564587457No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAG374110.11832553568548679No Hit
ACGTACGCAGTTTTATCCGGTAAAGC367200.11614000348483268No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG361000.11417903392708223No Hit
CACCACCGGCCCGTCTCGC353400.11177526479177523No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGG343600.10867566774887938No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG338890.10718596345581412No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC336280.1063604585290837TruSeq Adapter, Index 12 (100% over 50bp)
GGCGGTGCGCCGCGACCGGCTCC331500.10484861425714061No Hit
CGAATCCCGGGGCCGAGGAAGCCAG326230.10318179013305273No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGC317600.10045224702282914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)