FastQCFastQC Report
Tue 4 Jul 2023
SRR6869751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6869751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28044843
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG4143421.4774267055087453RNA PCR Primer, Index 1 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG2902531.034960331209556RNA PCR Primer, Index 1 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC2772740.9886808779781724RNA PCR Primer, Index 1 (100% over 44bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG2402620.8567065253315912RNA PCR Primer, Index 1 (100% over 45bp)
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC2292220.8173409991990328RNA PCR Primer, Index 1 (100% over 42bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC2094750.7469287669037763RNA PCR Primer, Index 1 (100% over 44bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG2013810.7180678458424602RNA PCR Primer, Index 1 (100% over 45bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT1984300.7075454121814838RNA PCR Primer, Index 1 (100% over 46bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG1839420.6558852905683943RNA PCR Primer, Index 1 (100% over 45bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1659700.5918022076286895RNA PCR Primer, Index 1 (100% over 44bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG1584250.564898865720161RNA PCR Primer, Index 1 (100% over 45bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC1573540.5610799817991493No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1571830.5604702440302483No Hit
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC1532220.5463464352430142RNA PCR Primer, Index 1 (100% over 38bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG1464150.5220745931792166RNA PCR Primer, Index 1 (100% over 45bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1462930.5216395755897082RNA PCR Primer, Index 1 (100% over 44bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT1437930.5127252807227339RNA PCR Primer, Index 1 (100% over 46bp)
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA1300580.4637501447235771RNA PCR Primer, Index 1 (100% over 37bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1137560.40562181075501114RNA PCR Primer, Index 1 (100% over 44bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG1118780.3989253924509401RNA PCR Primer, Index 1 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT1044500.37243923954218605RNA PCR Primer, Index 1 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1041700.3714408385170849RNA PCR Primer, Index 1 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1033700.3685882641596532RNA PCR Primer, Index 1 (100% over 44bp)
TCAAAGGCGGACATGCGACGCGCCAGTGTCGATGGAATTCTCGGGTGCCA1021250.3641489453159No Hit
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT890930.31768050903333633RNA PCR Primer, Index 1 (100% over 43bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC828280.29534128609669874RNA PCR Primer, Index 1 (100% over 44bp)
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT758280.27038126046917077RNA PCR Primer, Index 1 (100% over 43bp)
TAACGACCCGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA747260.2664518392918085RNA PCR Primer, Index 1 (100% over 34bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC692670.24698658502028342RNA PCR Primer, Index 1 (100% over 44bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA687980.24531426330323902RNA PCR Primer, Index 1 (100% over 40bp)
ACCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC685190.24431942799608472RNA PCR Primer, Index 1 (100% over 44bp)
CGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC661370.23582588784683162RNA PCR Primer, Index 1 (100% over 44bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC645970.23033468220877543RNA PCR Primer, Index 1 (100% over 44bp)
CGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT622770.22206221657222325RNA PCR Primer, Index 1 (100% over 43bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC590880.21069114203991088RNA PCR Primer, Index 1 (100% over 44bp)
ACGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG582830.20782073909274512RNA PCR Primer, Index 1 (100% over 45bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT574360.20480057599181425RNA PCR Primer, Index 1 (100% over 43bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG508120.18118126031227916RNA PCR Primer, Index 1 (100% over 45bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC503900.17967652733873388RNA PCR Primer, Index 1 (100% over 44bp)
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG492850.17573640900753126No Hit
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC474530.1692040137290125RNA PCR Primer, Index 1 (100% over 44bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT469620.16745324621713875No Hit
CGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG463850.16539582696184107RNA PCR Primer, Index 1 (100% over 45bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC460090.16405511701384815RNA PCR Primer, Index 1 (100% over 24bp)
ATTAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAA457210.1630281902451727RNA PCR Primer, Index 1 (100% over 23bp)
GCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG447850.15969067824697752RNA PCR Primer, Index 1 (100% over 45bp)
CCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG435800.1553939881210959RNA PCR Primer, Index 1 (100% over 45bp)
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC419780.14968170797033878RNA PCR Primer, Index 1 (100% over 44bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT416570.14853711250941928RNA PCR Primer, Index 1 (100% over 43bp)
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC402260.1434345701275632RNA PCR Primer, Index 1 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG396520.1413878480261059No Hit
AATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGAA394730.14074958451363054RNA PCR Primer, Index 1 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGA378300.13489110992705503RNA PCR Primer, Index 1 (100% over 22bp)
CTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG374800.13364310864567863RNA PCR Primer, Index 1 (100% over 45bp)
CCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT371050.1323059644156325RNA PCR Primer, Index 1 (100% over 43bp)
GGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC371000.13228813582589855RNA PCR Primer, Index 1 (100% over 44bp)
TCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC369630.13179963246718834RNA PCR Primer, Index 1 (100% over 44bp)
CGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG361590.12893279523796944RNA PCR Primer, Index 1 (100% over 45bp)
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC338600.12073520967829986RNA PCR Primer, Index 1 (100% over 44bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC335970.11979742585829416RNA PCR Primer, Index 1 (100% over 44bp)
GCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC326430.11639573093705678RNA PCR Primer, Index 1 (100% over 44bp)
CGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG325830.11618178786024938RNA PCR Primer, Index 1 (100% over 45bp)
AACGACCCGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT324080.11555778721956118RNA PCR Primer, Index 1 (100% over 35bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC317970.11337913355407266RNA PCR Primer, Index 1 (100% over 44bp)
CGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG314290.11206694934965404RNA PCR Primer, Index 1 (100% over 45bp)
CTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC312200.111321714298775RNA PCR Primer, Index 1 (100% over 44bp)
AGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG305230.10883640888986257RNA PCR Primer, Index 1 (100% over 45bp)
CCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC304880.10871160876172493RNA PCR Primer, Index 1 (100% over 44bp)
GGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG299570.1068182125319796RNA PCR Primer, Index 1 (100% over 45bp)
GCCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC299090.10664705807053368RNA PCR Primer, Index 1 (100% over 44bp)
ACGACCCGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC287850.10263919109834205RNA PCR Primer, Index 1 (100% over 36bp)
TAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACT282260.10064595476608658RNA PCR Primer, Index 1 (100% over 25bp)
GAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC282180.10061742902251226RNA PCR Primer, Index 1 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)