FastQCFastQC Report
Tue 4 Jul 2023
SRR6869752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6869752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19282869
Total Bases964.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG3275661.6987409912912854RNA PCR Primer, Index 2 (100% over 38bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG2741631.4217956881831224No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG1658280.8599757639799347RNA PCR Primer, Index 2 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG1495440.7755277495273136RNA PCR Primer, Index 2 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC1373960.7125288254564194RNA PCR Primer, Index 2 (100% over 44bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG1336250.6929726069289793RNA PCR Primer, Index 2 (100% over 45bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC1062540.55102796165861No Hit
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT992120.5145084997465885RNA PCR Primer, Index 2 (100% over 37bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG977430.5068903387768697RNA PCR Primer, Index 2 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG971480.5038046983568679RNA PCR Primer, Index 2 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT912250.47308831481456415RNA PCR Primer, Index 2 (100% over 43bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC825520.4281105679865377RNA PCR Primer, Index 2 (100% over 44bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC822100.4263369729888224RNA PCR Primer, Index 2 (100% over 44bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT742400.3850049492116552RNA PCR Primer, Index 2 (100% over 46bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG742040.38481825500136935RNA PCR Primer, Index 2 (100% over 45bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT714620.37059837931793244RNA PCR Primer, Index 2 (100% over 46bp)
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC685360.3554242887819235RNA PCR Primer, Index 2 (100% over 42bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC667410.3461155080190609RNA PCR Primer, Index 2 (100% over 44bp)
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA611480.3171104880710438RNA PCR Primer, Index 1 (100% over 28bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG564720.2928609845350295RNA PCR Primer, Index 2 (100% over 45bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTG558820.28980127386645627No Hit
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG555070.28785654250931225RNA PCR Primer, Index 2 (100% over 45bp)
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG525320.2724283404093032RNA PCR Primer, Index 1 (100% over 29bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC503720.26122668779215374RNA PCR Primer, Index 2 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC485180.2516119359624338RNA PCR Primer, Index 2 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC450310.23352852731613744RNA PCR Primer, Index 2 (100% over 44bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC446870.23174456041785066RNA PCR Primer, Index 2 (100% over 44bp)
AAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGA446500.23155268025727913RNA PCR Primer, Index 1 (100% over 22bp)
AATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGAA412400.213868589782983RNA PCR Primer, Index 1 (100% over 23bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA403820.20941904443783754RNA PCR Primer, Index 2 (100% over 40bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT344370.17858856998924796RNA PCR Primer, Index 2 (100% over 43bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGG342930.1778417931481047No Hit
CGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT333110.17274918996753025RNA PCR Primer, Index 2 (100% over 43bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA326000.1690619793143852No Hit
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT323600.16781735124581307RNA PCR Primer, Index 2 (100% over 43bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC322710.16735580167038422RNA PCR Primer, Index 2 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG306770.15908939691495078No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA295700.15334854994866168No Hit
ACCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC292740.15181350866408935RNA PCR Primer, Index 2 (100% over 44bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT277920.14412793034065627RNA PCR Primer, Index 2 (100% over 43bp)
CGGCGGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA277260.1437856576217989RNA PCR Primer, Index 2 (100% over 40bp)
TAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCC272770.14145716594351185RNA PCR Primer, Index 1 (100% over 27bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC271920.14101636016922586RNA PCR Primer, Index 2 (100% over 44bp)
TAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGG268390.13918571971836763Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC257990.1337923314212216RNA PCR Primer, Index 2 (100% over 44bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC253640.13153644304693457RNA PCR Primer, Index 2 (100% over 44bp)
CGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC249060.129161277816076RNA PCR Primer, Index 2 (100% over 44bp)
ACGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG247410.12830559601893265RNA PCR Primer, Index 2 (100% over 45bp)
GGGGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC242130.1255674142680739RNA PCR Primer, Index 2 (100% over 42bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC229500.11901755905721291RNA PCR Primer, Index 2 (100% over 44bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAAC223750.11603563764292543RNA PCR Primer, Index 1 (100% over 24bp)
GGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC223700.11600970789149684RNA PCR Primer, Index 2 (100% over 44bp)
CGGCGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT222400.1153355343543536RNA PCR Primer, Index 2 (100% over 41bp)
GTGAGACGAATTTTTGAGCGGGTAAAGGTCGCTGGAATTCTCGGGTGCCA217120.11259735260349484No Hit
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT211590.10972952209549315RNA PCR Primer, Index 2 (100% over 43bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC206290.10698096844406298RNA PCR Primer, Index 1 (100% over 26bp)
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC204850.10623419160291968RNA PCR Primer, Index 2 (100% over 44bp)
AAATGGATTTTTGGAGCAGGGAGTGGAATTCTCGGGTGCCAAGGAACTCC202850.1051970015457762RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC196570.10194022476634572RNA PCR Primer, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)