FastQCFastQC Report
Tue 4 Jul 2023
SRR6869753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6869753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24306647
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG5233732.1532093669686323No Hit
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG1530500.6296631534575707RNA PCR Primer, Index 3 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC1510350.6213732400030328RNA PCR Primer, Index 3 (100% over 44bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG1377110.5665569586788338RNA PCR Primer, Index 3 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT1358820.559032268004715RNA PCR Primer, Index 3 (100% over 43bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG1192120.49045020483491614RNA PCR Primer, Index 3 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1152030.4739567740462105No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC1121980.46159390063137873No Hit
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG965330.3971465089364239RNA PCR Primer, Index 3 (100% over 45bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT859920.3537797706117179RNA PCR Primer, Index 3 (100% over 46bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC857910.3529528363167491RNA PCR Primer, Index 3 (100% over 44bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC853960.3513277664336015RNA PCR Primer, Index 3 (100% over 44bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT746520.30712586561198674RNA PCR Primer, Index 3 (100% over 46bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG704820.289970064567112RNA PCR Primer, Index 3 (100% over 45bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG694230.28561323163988844RNA PCR Primer, Index 3 (100% over 45bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG664690.27346017737452644RNA PCR Primer, Index 3 (100% over 45bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG591340.24328324675962093No Hit
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC585810.2410081489232143RNA PCR Primer, Index 3 (100% over 44bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTG538690.22162250515260293No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGG533370.21943380343656615No Hit
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC518780.21343133012134502RNA PCR Primer, Index 3 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC470210.19344914171008448RNA PCR Primer, Index 3 (100% over 44bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG447820.1842376696382681RNA PCR Primer, Index 3 (100% over 45bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG438320.18032927371677385No Hit
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT429100.17653607262243945RNA PCR Primer, Index 3 (100% over 43bp)
AAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGA413400.170076934099549RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG412170.16957089968023972No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA411620.16934462412689005No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC400760.16487671047347666RNA PCR Primer, Index 3 (100% over 44bp)
AATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGAA397790.16365482248538846RNA PCR Primer, Index 1 (100% over 23bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA382120.15720802626540797RNA PCR Primer, Index 3 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG362150.1489921666283301No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC359650.14796364138583162RNA PCR Primer, Index 1 (100% over 26bp)
TAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACT353320.14535941547182546RNA PCR Primer, Index 1 (100% over 25bp)
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT349640.14384542631486769RNA PCR Primer, Index 3 (100% over 43bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC346880.14270993444714936RNA PCR Primer, Index 3 (100% over 44bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA321390.13222309107463484No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG312540.12858211171619022Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG312420.1285327425045503RNA PCR Primer, Index 3 (100% over 38bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT299810.12334486118138796RNA PCR Primer, Index 3 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC292690.12041562129075228RNA PCR Primer, Index 3 (100% over 44bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC289020.1189057462347645RNA PCR Primer, Index 3 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC282070.11604644606061873RNA PCR Primer, Index 3 (100% over 44bp)
TAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGG279230.11487804138514046Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA277410.11412927500860155No Hit
ATTAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAA270080.11111363899759602RNA PCR Primer, Index 1 (100% over 23bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT250050.10287309475469816RNA PCR Primer, Index 3 (100% over 43bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG250020.10286075245178818RNA PCR Primer, Index 3 (100% over 45bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA246480.10140436070841033No Hit
CTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG245980.10119865565991062RNA PCR Primer, Index 3 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)