FastQCFastQC Report
Tue 4 Jul 2023
SRR6869754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6869754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18003822
Total Bases900.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC3467101.9257577641014225RNA PCR Primer, Index 4 (100% over 38bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG2748921.5268535758685018No Hit
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC1754950.9747652470680948RNA PCR Primer, Index 4 (100% over 37bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG1276870.7092216308292761RNA PCR Primer, Index 4 (100% over 45bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC1015430.5640080200748485No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG898540.4990829169495232RNA PCR Primer, Index 4 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG845000.469344786901359RNA PCR Primer, Index 4 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC716900.3981932280823483RNA PCR Primer, Index 4 (100% over 44bp)
AAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGA687370.3817911552335943RNA PCR Primer, Index 1 (100% over 22bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG685720.38087468316449696RNA PCR Primer, Index 4 (100% over 45bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG664780.3692438194512254RNA PCR Primer, Index 4 (100% over 45bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT622810.3459321026390952RNA PCR Primer, Index 4 (100% over 46bp)
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC593100.32943005101916695RNA PCR Primer, Index 4 (100% over 42bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTG581570.32302585528783834No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA579980.3221427094757991No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT555390.3084844984581607RNA PCR Primer, Index 4 (100% over 43bp)
AATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGAA538730.29923090774836586RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA517400.2873834233642168No Hit
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC452240.25119110819913687RNA PCR Primer, Index 4 (100% over 44bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT442950.24603109273131007RNA PCR Primer, Index 4 (100% over 46bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGG441330.24513128379074178No Hit
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG434340.2412487748434749RNA PCR Primer, Index 4 (100% over 45bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC418550.2324784148610223RNA PCR Primer, Index 4 (100% over 44bp)
TAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGG407950.226590776114094Illumina Small RNA Adapter 2 (100% over 21bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG400660.22254163588153672RNA PCR Primer, Index 4 (100% over 45bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC391980.21772043736046715RNA PCR Primer, Index 4 (100% over 44bp)
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA379270.2106608252403295RNA PCR Primer, Index 1 (100% over 28bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC359460.19965760603498522RNA PCR Primer, Index 4 (100% over 44bp)
CGGCGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT350250.19454202557656924RNA PCR Primer, Index 4 (100% over 41bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG346650.19254245015308416RNA PCR Primer, Index 4 (100% over 45bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC343490.19078726728135836RNA PCR Primer, Index 4 (100% over 44bp)
CGGCGGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA338390.18795453543142118RNA PCR Primer, Index 4 (100% over 40bp)
CGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT324930.18047834509805752RNA PCR Primer, Index 4 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG313400.1740741493667289No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC310520.17247448902794085RNA PCR Primer, Index 4 (100% over 50bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA301670.16755886611187334RNA PCR Primer, Index 4 (100% over 40bp)
ATTAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAA288730.16037150333967976No Hit
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC284670.15811642661208272RNA PCR Primer, Index 4 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC283060.15722217204769076RNA PCR Primer, Index 4 (100% over 44bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC277280.15401174261776193No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACT273370.1518399815328101RNA PCR Primer, Index 1 (100% over 25bp)
ATTAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAA273120.15170112212840142RNA PCR Primer, Index 1 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC265460.14744646997731925RNA PCR Primer, Index 1 (100% over 26bp)
TTAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAG255040.14165881000156524No Hit
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG251190.13952037517367147RNA PCR Primer, Index 1 (100% over 29bp)
TAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCC239950.13327725635145693RNA PCR Primer, Index 1 (100% over 27bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC237850.13211083735442397No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG237810.13208861984971856No Hit
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC235650.13088887459562754RNA PCR Primer, Index 4 (100% over 44bp)
TTAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAAC227000.126084339203087RNA PCR Primer, Index 1 (100% over 24bp)
GTGAGACGAATTTTTGAGCGGGTAAAGGTCGCTGGAATTCTCGGGTGCCA224520.12470685391135282No Hit
AAATGGATTTTTGGAGCAGGGAGTGGAATTCTCGGGTGCCAAGGAACTCC203630.11310376207896301RNA PCR Primer, Index 1 (100% over 27bp)
ATTAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACT192380.10685508888057214RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC192030.10666068571439997RNA PCR Primer, Index 1 (100% over 24bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT190980.1060774762158835RNA PCR Primer, Index 4 (100% over 43bp)
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC190850.10600526932559098RNA PCR Primer, Index 4 (100% over 44bp)
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC189920.10548871234119066RNA PCR Primer, Index 4 (100% over 44bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT188870.10490550284267418RNA PCR Primer, Index 4 (100% over 43bp)
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT186050.1033391687609442RNA PCR Primer, Index 4 (100% over 43bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC185990.10330584250388612RNA PCR Primer, Index 4 (100% over 44bp)
CTGTCCCCGCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA180650.10033980562571658RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)