FastQCFastQC Report
Fri 7 Jul 2023
SRR6869755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6869755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18790023
Total Bases939.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG7073603.7645510066698695No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC7073503.7644977869372487No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG2850671.5171189519033585RNA PCR Primer, Index 5 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC1569510.8352890254578188RNA PCR Primer, Index 5 (100% over 44bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1381300.7351241666920791RNA PCR Primer, Index 5 (100% over 45bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1344590.7155872028469576RNA PCR Primer, Index 5 (100% over 45bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT1208560.643192400562788RNA PCR Primer, Index 5 (100% over 46bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1098690.5847198803322381RNA PCR Primer, Index 5 (100% over 45bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGG1088160.5791158424872604No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT1029170.5477215222142091RNA PCR Primer, Index 5 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1014830.5400898125563763RNA PCR Primer, Index 5 (100% over 50bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC928580.49418779317087586RNA PCR Primer, Index 5 (100% over 44bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC903250.4807072348980095RNA PCR Primer, Index 5 (100% over 44bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC852450.45367161072660744RNA PCR Primer, Index 5 (100% over 44bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG792060.421532214196864RNA PCR Primer, Index 5 (100% over 45bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG754520.4015535265709893RNA PCR Primer, Index 5 (100% over 45bp)
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGT721530.38399633677936423RNA PCR Primer, Index 5 (100% over 38bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC719610.3829745179130435RNA PCR Primer, Index 5 (100% over 44bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG693670.36916931927118984RNA PCR Primer, Index 5 (100% over 45bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC667090.35502351434056256RNA PCR Primer, Index 5 (100% over 44bp)
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC631570.3361198653136295RNA PCR Primer, Index 5 (100% over 42bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTG623750.3319580822226774No Hit
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC593370.31578992745245704RNA PCR Primer, Index 5 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG558830.2974078318052085No Hit
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGA542660.2888022010404139RNA PCR Primer, Index 5 (100% over 40bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC538610.2866468018692686RNA PCR Primer, Index 5 (100% over 44bp)
GTGAGACGAATTTTTGAGCGGGTAAAGGTCGCTGGAATTCTCGGGTGCCA535880.28519390316871884No Hit
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT501380.26683309541451866RNA PCR Primer, Index 5 (100% over 46bp)
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC469370.24979745900257813RNA PCR Primer, Index 5 (100% over 44bp)
AAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGA461430.24557181223248103RNA PCR Primer, Index 1 (100% over 22bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG458070.24378362921641977RNA PCR Primer, Index 5 (100% over 45bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT425040.2262051515317464RNA PCR Primer, Index 5 (100% over 43bp)
CGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT421370.22425198734456045RNA PCR Primer, Index 5 (100% over 43bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT418930.22295342586861125RNA PCR Primer, Index 5 (100% over 43bp)
CGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC392580.20893002632301197RNA PCR Primer, Index 5 (100% over 44bp)
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT389350.20721102895935786RNA PCR Primer, Index 5 (100% over 43bp)
ATGTACACGGAGTCGACCCAACGCGATGGAATTCTCGGGTGCCAAGGAAC388580.20680123701817715RNA PCR Primer, Index 1 (100% over 24bp)
AATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGAA387810.20639144507699644RNA PCR Primer, Index 1 (100% over 23bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC381020.20277782523203938RNA PCR Primer, Index 5 (100% over 44bp)
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA369330.19655643848865964RNA PCR Primer, Index 1 (100% over 28bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA355640.18927065709286253No Hit
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC353320.18803595929605835RNA PCR Primer, Index 5 (100% over 44bp)
GGGGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC333190.1773228271194772RNA PCR Primer, Index 5 (100% over 42bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC328280.17470973824779246RNA PCR Primer, Index 5 (100% over 44bp)
ACCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC313610.1669024034723108RNA PCR Primer, Index 5 (100% over 44bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG313590.16689175952578664RNA PCR Primer, Index 5 (100% over 45bp)
TAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGG312660.16639681601241255Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC311820.16594977025839724RNA PCR Primer, Index 5 (100% over 44bp)
TCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGG298890.15906845883051873Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC293530.15621588116204008RNA PCR Primer, Index 5 (100% over 44bp)
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC292010.15540694122620288RNA PCR Primer, Index 5 (100% over 44bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC278880.1484191903330826RNA PCR Primer, Index 5 (100% over 44bp)
CGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG276210.1469982234721054RNA PCR Primer, Index 5 (100% over 45bp)
ATTAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAA273710.14566773015658363RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA272500.14502377139187111No Hit
ATTAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAA240740.12812118431148276No Hit
CGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG240570.12803071076602726RNA PCR Primer, Index 5 (100% over 45bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC239910.12767946053072954RNA PCR Primer, Index 1 (100% over 26bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT234290.12468851155743661RNA PCR Primer, Index 5 (100% over 43bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG228730.12172949442371625Illumina Small RNA Adapter 2 (100% over 21bp)
TAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCC217890.11596047540761392RNA PCR Primer, Index 1 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA217770.11589661172846889RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC217010.11549214176055027No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACT216310.11511960363220416RNA PCR Primer, Index 1 (100% over 25bp)
ACGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG216300.11511428165894208RNA PCR Primer, Index 5 (100% over 45bp)
AGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG214350.11407649687283512RNA PCR Primer, Index 5 (100% over 45bp)
TCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC207270.11030853980327751RNA PCR Primer, Index 5 (100% over 44bp)
CCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT203530.10831812180325698RNA PCR Primer, Index 5 (100% over 43bp)
AAATGGATTTTTGGAGCAGGGAGTGGAATTCTCGGGTGCCAAGGAACTCC203430.1082649020706361RNA PCR Primer, Index 1 (100% over 27bp)
GCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC196330.10448630105455432RNA PCR Primer, Index 5 (100% over 44bp)
GGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC192490.10244266332191292RNA PCR Primer, Index 5 (100% over 44bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG191270.10179338258393829No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC190160.10120264355184665No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC189280.10073430990478298No Hit

[FAIL]Adapter Content

Adapter graph