FastQCFastQC Report
Tue 4 Jul 2023
SRR6869756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6869756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22562947
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG6011812.664461340090016No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC4719602.0917480327370357No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG2606401.155168249963092RNA PCR Primer, Index 6 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG2599781.1522342360685418RNA PCR Primer, Index 6 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG2145070.950704710692269RNA PCR Primer, Index 6 (100% over 45bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT1749790.7755148296895791RNA PCR Primer, Index 6 (100% over 46bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC1661370.7363266864031547RNA PCR Primer, Index 6 (100% over 44bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG1634150.724262659483267RNA PCR Primer, Index 6 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG1491680.6611193121182264RNA PCR Primer, Index 6 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT1359240.6024213060465905RNA PCR Primer, Index 6 (100% over 43bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG1142890.5065340090547569RNA PCR Primer, Index 6 (100% over 45bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGG1140750.5055855513909597No Hit
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC1038930.4604584675929079RNA PCR Primer, Index 6 (100% over 44bp)
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA974510.4319072326855175RNA PCR Primer, Index 6 (100% over 38bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC962780.4267084437152647RNA PCR Primer, Index 6 (100% over 44bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT946350.4194265935207843RNA PCR Primer, Index 6 (100% over 46bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC921060.4082179513163773RNA PCR Primer, Index 6 (100% over 44bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG918800.4072163091106849RNA PCR Primer, Index 6 (100% over 45bp)
GTGAGACGAATTTTTGAGCGGGTAAAGGTCGCTGGAATTCTCGGGTGCCA885730.39255953577340763No Hit
AAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGA836890.37091342722207343RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC835570.3703283972612265RNA PCR Primer, Index 6 (100% over 42bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC826370.3662509157159302RNA PCR Primer, Index 6 (100% over 44bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG788280.34936925570937166RNA PCR Primer, Index 6 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG695800.3083817020888273No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTG679990.30137463869413866No Hit
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC658310.2917659647917446RNA PCR Primer, Index 6 (100% over 44bp)
AATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGAA637140.28238332519240505RNA PCR Primer, Index 1 (100% over 23bp)
TAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGG600960.2663481858110113Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC595630.26398590574183417RNA PCR Primer, Index 6 (100% over 44bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC570480.2528393121696381RNA PCR Primer, Index 6 (100% over 44bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA544670.24140020361701864RNA PCR Primer, Index 6 (100% over 40bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC527300.2337017411776928RNA PCR Primer, Index 6 (100% over 44bp)
CGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC476740.2112933208591945RNA PCR Primer, Index 6 (100% over 44bp)
ATTAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAA463630.2054829096571472RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC450480.19965477027446815RNA PCR Primer, Index 6 (100% over 44bp)
ATTAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAA441860.19583434734833177No Hit
CGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT438340.1942742674527401RNA PCR Primer, Index 6 (100% over 43bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC432550.19170811330629817RNA PCR Primer, Index 6 (100% over 44bp)
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA431560.191269340835663RNA PCR Primer, Index 1 (100% over 28bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC430190.19066215064902647RNA PCR Primer, Index 6 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC424580.18817577331542726RNA PCR Primer, Index 6 (100% over 50bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT418920.18566723575603844RNA PCR Primer, Index 6 (100% over 43bp)
ACCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC415000.18392987405412956RNA PCR Primer, Index 6 (100% over 44bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG410800.1820684150877986RNA PCR Primer, Index 6 (100% over 45bp)
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT408650.18111552537884346RNA PCR Primer, Index 6 (100% over 43bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT402160.17823912807134634RNA PCR Primer, Index 6 (100% over 43bp)
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC394420.1748087251191079RNA PCR Primer, Index 6 (100% over 44bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGGAATTCTC392200.17382481109404724No Hit
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC383980.1701816699742281RNA PCR Primer, Index 6 (100% over 44bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA381220.1689584255106392No Hit
ACGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG369030.1635557624631215RNA PCR Primer, Index 6 (100% over 45bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC336360.14907627093216147RNA PCR Primer, Index 6 (100% over 44bp)
TAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACT326330.14463092963875684RNA PCR Primer, Index 1 (100% over 25bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC320580.14208250367294661RNA PCR Primer, Index 1 (100% over 26bp)
CGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG313120.13877619798513022RNA PCR Primer, Index 6 (100% over 45bp)
GGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC312740.13860778026912884RNA PCR Primer, Index 6 (100% over 44bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG309540.13718952581859098No Hit
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA304970.1351640811814166RNA PCR Primer, Index 6 (100% over 37bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC303040.13430869646593593RNA PCR Primer, Index 6 (100% over 44bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT291650.12926059703105272RNA PCR Primer, Index 6 (100% over 43bp)
TAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCC286690.12706230263271903RNA PCR Primer, Index 1 (100% over 27bp)
CTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG285760.12665012243303148RNA PCR Primer, Index 6 (100% over 45bp)
TTAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAG279720.12397316715764126No Hit
AAATGGATTTTTGGAGCAGGGAGTGGAATTCTCGGGTGCCAAGGAACTCC272950.12097267258572206RNA PCR Primer, Index 1 (100% over 27bp)
CGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG266660.11818491618138358RNA PCR Primer, Index 6 (100% over 45bp)
GGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG266100.11793672165253946RNA PCR Primer, Index 6 (100% over 45bp)
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG255990.11345592399787138RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC255140.11307920015944727RNA PCR Primer, Index 6 (100% over 44bp)
CCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT245450.10878454840141229RNA PCR Primer, Index 6 (100% over 43bp)
TATTAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGA241100.10685660875771237RNA PCR Primer, Index 1 (100% over 22bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC240950.10679012808034341RNA PCR Primer, Index 6 (100% over 44bp)
CCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG239530.10616077766791723RNA PCR Primer, Index 6 (100% over 45bp)
GCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG238060.1055092670297014RNA PCR Primer, Index 6 (100% over 45bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC236510.10482230003022212RNA PCR Primer, Index 6 (100% over 44bp)
CGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG234900.10410874075979526RNA PCR Primer, Index 6 (100% over 45bp)
TTAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAAC232470.10303175378641805RNA PCR Primer, Index 1 (100% over 24bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA228530.10128552799419331RNA PCR Primer, Index 6 (100% over 37bp)
TCGGAAGCATAATTTTTTGGTATTTGGGCTGGAATTCTCGGGTGCCAAGG227510.10083345938808437Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)