FastQCFastQC Report
Tue 4 Jul 2023
SRR6869757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6869757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20558434
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT4241632.063206759814488RNA PCR Primer, Index 7 (100% over 38bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG3506531.705640614455362RNA PCR Primer, Index 7 (100% over 45bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC2226161.0828451233201908RNA PCR Primer, Index 7 (100% over 44bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC2178971.0598910403389674RNA PCR Primer, Index 7 (100% over 44bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG2083731.013564554576482RNA PCR Primer, Index 7 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG1868090.9086732968085021RNA PCR Primer, Index 7 (100% over 45bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG1749890.8511786452217128RNA PCR Primer, Index 7 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG1632820.7942336463954404RNA PCR Primer, Index 7 (100% over 45bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC1590010.7734100758841846RNA PCR Primer, Index 7 (100% over 44bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG1256100.6109901172433659RNA PCR Primer, Index 7 (100% over 45bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1229990.5982897335468256No Hit
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT1144950.5569247151801543RNA PCR Primer, Index 7 (100% over 46bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC1113300.5415295737019659RNA PCR Primer, Index 7 (100% over 44bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC1105440.537706325296956RNA PCR Primer, Index 7 (100% over 44bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT1070570.5207449166604811RNA PCR Primer, Index 7 (100% over 43bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG1023480.4978394755164717RNA PCR Primer, Index 7 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC1005370.48903043879704067RNA PCR Primer, Index 7 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC898370.4369836729782045RNA PCR Primer, Index 7 (100% over 44bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC893750.4347364200989239RNA PCR Primer, Index 7 (100% over 44bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT869510.42294563875828284RNA PCR Primer, Index 7 (100% over 43bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG858580.41762908595080733RNA PCR Primer, Index 7 (100% over 45bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT781180.3799803039472754RNA PCR Primer, Index 7 (100% over 46bp)
ACCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC780640.37971763802632047RNA PCR Primer, Index 7 (100% over 44bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA701690.3413149075459736RNA PCR Primer, Index 7 (100% over 40bp)
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT643690.31310264196193155RNA PCR Primer, Index 7 (100% over 43bp)
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC614810.29905487937456715RNA PCR Primer, Index 7 (100% over 42bp)
CGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC602100.29287250186468483RNA PCR Primer, Index 7 (100% over 44bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG566570.2755900571025984RNA PCR Primer, Index 7 (100% over 45bp)
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC564740.2746999114815846RNA PCR Primer, Index 7 (100% over 44bp)
ACGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG560410.27259371993022424RNA PCR Primer, Index 7 (100% over 45bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTG541700.26349283218750996No Hit
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC495030.2407916867597989RNA PCR Primer, Index 7 (100% over 44bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC459690.2236016614884188RNA PCR Primer, Index 7 (100% over 44bp)
CGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT423800.20614410611236247RNA PCR Primer, Index 7 (100% over 43bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC414570.20165446453752267RNA PCR Primer, Index 7 (100% over 44bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC412290.2005454306490465No Hit
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT410700.19977202543734604RNA PCR Primer, Index 7 (100% over 43bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT410170.1995142237001126RNA PCR Primer, Index 7 (100% over 43bp)
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA402110.195593691620675RNA PCR Primer, Index 1 (100% over 23bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC401980.19553045723229695RNA PCR Primer, Index 7 (100% over 44bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG378800.18425527936612293Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC378470.18409476130331717RNA PCR Primer, Index 7 (100% over 42bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA374560.18219286546825503RNA PCR Primer, Index 1 (100% over 22bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC356960.17363190211861468RNA PCR Primer, Index 7 (100% over 44bp)
AATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGAA354920.17263960863945182RNA PCR Primer, Index 1 (100% over 23bp)
GCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC334650.16277990823620128RNA PCR Primer, Index 7 (100% over 44bp)
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC321010.15614516164022998RNA PCR Primer, Index 7 (100% over 44bp)
TCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC320480.15588735990299651RNA PCR Primer, Index 7 (100% over 44bp)
GGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC308900.15025463515363088RNA PCR Primer, Index 7 (100% over 44bp)
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC307420.14953473596286565RNA PCR Primer, Index 7 (100% over 44bp)
CCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT307000.14933044024656741RNA PCR Primer, Index 7 (100% over 43bp)
GCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG305010.1483624676860115RNA PCR Primer, Index 7 (100% over 36bp)
AAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGA298650.14526884683920963RNA PCR Primer, Index 1 (100% over 22bp)
GAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC290960.14152828955746335RNA PCR Primer, Index 7 (100% over 44bp)
GCCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC288700.14042898403643RNA PCR Primer, Index 7 (100% over 44bp)
GCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG286960.13958261606890873RNA PCR Primer, Index 7 (100% over 45bp)
CGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG284640.13845412544554708RNA PCR Primer, Index 7 (100% over 45bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC282880.13759802911058303RNA PCR Primer, Index 7 (100% over 44bp)
ACCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT272890.13273870957291786RNA PCR Primer, Index 7 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG267320.13002935924010553No Hit
CGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG260490.12670712175839852RNA PCR Primer, Index 7 (100% over 45bp)
AGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG257130.12507275602801265RNA PCR Primer, Index 7 (100% over 45bp)
CTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC250400.12179916038351948RNA PCR Primer, Index 7 (100% over 44bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAA250370.12178456783235533No Hit
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA250250.12172619762769868RNA PCR Primer, Index 1 (100% over 28bp)
ACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT245400.11936706852282622RNA PCR Primer, Index 7 (100% over 43bp)
CGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG244580.11896820545767252RNA PCR Primer, Index 7 (100% over 45bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC243250.11832126902272808RNA PCR Primer, Index 1 (100% over 24bp)
CCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC241050.11725114860402304RNA PCR Primer, Index 7 (100% over 44bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC240410.1169398408458543RNA PCR Primer, Index 7 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC238380.11595241155041283RNA PCR Primer, Index 7 (100% over 50bp)
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG233770.11371002285485363RNA PCR Primer, Index 1 (100% over 29bp)
TCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC233420.1135397764246051RNA PCR Primer, Index 7 (100% over 44bp)
CCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC225840.10985272516379409RNA PCR Primer, Index 7 (100% over 44bp)
CGGCGGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA223740.10883124658230292RNA PCR Primer, Index 7 (100% over 40bp)
GGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG220040.10703149860538988RNA PCR Primer, Index 7 (100% over 45bp)
CCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC219230.10663749972395759RNA PCR Primer, Index 7 (100% over 44bp)
CCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG219090.10656940115185817RNA PCR Primer, Index 7 (100% over 45bp)
CTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG212150.1031936576492159RNA PCR Primer, Index 7 (100% over 45bp)
CCCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT211630.10294072009570379RNA PCR Primer, Index 7 (100% over 43bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC208050.1011993423234474RNA PCR Primer, Index 7 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph