FastQCFastQC Report
Tue 4 Jul 2023
SRR6869758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6869758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25803528
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG4980051.9299880233431643RNA PCR Primer, Index 8 (100% over 45bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG4293971.664101901104376No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC3202261.2410163447416958RNA PCR Primer, Index 8 (100% over 44bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG3189001.2358775125633983RNA PCR Primer, Index 8 (100% over 45bp)
CGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT2884271.1177812584387685RNA PCR Primer, Index 8 (100% over 46bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG2552710.9892872013470406RNA PCR Primer, Index 8 (100% over 45bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC2386710.9249549131421099No Hit
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC2349130.9103910131978852RNA PCR Primer, Index 8 (100% over 44bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT2238410.867482152053006RNA PCR Primer, Index 8 (100% over 46bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG1986710.7699373512025177RNA PCR Primer, Index 8 (100% over 45bp)
CGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG1913030.7413831162932449RNA PCR Primer, Index 8 (100% over 45bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC1901790.7370271228027424RNA PCR Primer, Index 8 (100% over 44bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG1807570.7005127360878713RNA PCR Primer, Index 8 (100% over 45bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC1772700.6869990801257875RNA PCR Primer, Index 8 (100% over 44bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG1748340.6775585106036662RNA PCR Primer, Index 8 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT1158760.44907037518280446RNA PCR Primer, Index 8 (100% over 43bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG1144340.44348199207488215RNA PCR Primer, Index 8 (100% over 45bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC1028750.3986857921133885RNA PCR Primer, Index 8 (100% over 44bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT1019390.39505838116400205RNA PCR Primer, Index 8 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC979070.3794326109204912RNA PCR Primer, Index 8 (100% over 44bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC942040.36508186012393345RNA PCR Primer, Index 8 (100% over 44bp)
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTG934750.36225666505758436RNA PCR Primer, Index 8 (100% over 38bp)
GAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC919770.35645125736294664RNA PCR Primer, Index 8 (100% over 44bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTG880600.3412711626100121No Hit
ACGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG781320.3027958037366053RNA PCR Primer, Index 8 (100% over 45bp)
GCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT770680.29867233658901216RNA PCR Primer, Index 8 (100% over 43bp)
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC694960.26932751211384737RNA PCR Primer, Index 8 (100% over 42bp)
CGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT694710.26923062613763515RNA PCR Primer, Index 8 (100% over 43bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC693280.2686764383537011RNA PCR Primer, Index 8 (100% over 44bp)
ACCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC692210.2682617663755127RNA PCR Primer, Index 8 (100% over 44bp)
CGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC691320.2679168523001971RNA PCR Primer, Index 8 (100% over 44bp)
CGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC664300.25744541599117765RNA PCR Primer, Index 8 (100% over 44bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGG625680.24247847038591No Hit
CGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG548520.21257558268776272RNA PCR Primer, Index 8 (100% over 45bp)
AATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGAA529010.2050146011041591RNA PCR Primer, Index 1 (100% over 23bp)
GAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG519450.20130968137380287RNA PCR Primer, Index 8 (100% over 45bp)
CGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG506770.19639562466031776RNA PCR Primer, Index 8 (100% over 45bp)
CGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG506090.19613209480502047RNA PCR Primer, Index 8 (100% over 45bp)
AGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT498010.19300074005384069RNA PCR Primer, Index 8 (100% over 46bp)
AAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCA486070.18837346582994388RNA PCR Primer, Index 1 (100% over 28bp)
CTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG483900.1875324955564216RNA PCR Primer, Index 8 (100% over 45bp)
CGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG472610.18315712487067662RNA PCR Primer, Index 8 (100% over 45bp)
GCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG470490.18233553179239675RNA PCR Primer, Index 8 (100% over 45bp)
CCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG469030.18176971769131725RNA PCR Primer, Index 8 (100% over 45bp)
GGGGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC468220.18145580712838957RNA PCR Primer, Index 8 (100% over 42bp)
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC457750.17739822244462075RNA PCR Primer, Index 8 (100% over 44bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA425670.16496581397706547No Hit
GTGAGACGAATTTTTGAGCGGGTAAAGGTCGCTGGAATTCTCGGGTGCCA422080.16357453135865763No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG419050.16240027332696522No Hit
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC414250.1605400625836901RNA PCR Primer, Index 8 (100% over 44bp)
GCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAA413520.16025715553315034RNA PCR Primer, Index 8 (100% over 40bp)
TCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC411440.15945106421106447RNA PCR Primer, Index 8 (100% over 44bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA401790.1557112655292718No Hit
TAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCC399300.15474628120619785RNA PCR Primer, Index 1 (100% over 27bp)
AATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG391280.15163817908930902RNA PCR Primer, Index 1 (100% over 29bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT387120.15002599644513726No Hit
AGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG385380.14935167005070005RNA PCR Primer, Index 8 (100% over 45bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT384460.14899512965823897RNA PCR Primer, Index 8 (100% over 43bp)
CGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT382660.1482975506295108RNA PCR Primer, Index 8 (100% over 43bp)
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC378950.1468597627425211RNA PCR Primer, Index 8 (100% over 44bp)
GGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC372070.14419346067716013RNA PCR Primer, Index 8 (100% over 44bp)
AAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGGA366640.14208909727383015RNA PCR Primer, Index 1 (100% over 22bp)
GGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT362030.14030251987247636RNA PCR Primer, Index 8 (100% over 46bp)
GGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC361480.14008937072480943RNA PCR Primer, Index 8 (100% over 44bp)
CTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC361310.1400234882609851RNA PCR Primer, Index 8 (100% over 44bp)
CCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT343370.13307095060799437RNA PCR Primer, Index 8 (100% over 43bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC338660.13124561881615568RNA PCR Primer, Index 8 (100% over 44bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC335070.12985433619774783RNA PCR Primer, Index 8 (100% over 44bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC333220.12913737997377722RNA PCR Primer, Index 8 (100% over 44bp)
GGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG325120.1259982743445005RNA PCR Primer, Index 8 (100% over 45bp)
GGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC323490.12536657777959664RNA PCR Primer, Index 8 (100% over 44bp)
AGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT319800.12393654077070393RNA PCR Primer, Index 8 (100% over 46bp)
TATTAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAAC319270.12373114250113396RNA PCR Primer, Index 1 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC307730.11925888583917672RNA PCR Primer, Index 1 (100% over 26bp)
ATTAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACT307500.11916975074106147RNA PCR Primer, Index 1 (100% over 25bp)
GCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC296530.11491839410486815RNA PCR Primer, Index 8 (100% over 44bp)
TCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC296200.11479050461626798RNA PCR Primer, Index 8 (100% over 44bp)
CGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG295980.11470524495720119RNA PCR Primer, Index 8 (100% over 45bp)
GAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC295630.11456960459050405RNA PCR Primer, Index 8 (100% over 44bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT292320.11328683426545393RNA PCR Primer, Index 8 (100% over 43bp)
CGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC286850.11116696910593RNA PCR Primer, Index 8 (100% over 44bp)
TAAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACT286250.11093444276302061RNA PCR Primer, Index 1 (100% over 25bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGGGTGC285910.11080267783537195No Hit
ACCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT285200.1105275216629292RNA PCR Primer, Index 8 (100% over 43bp)
GCCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC280500.108706065310139RNA PCR Primer, Index 8 (100% over 44bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTC277620.10758993886417392No Hit
CGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT277540.107558935351786RNA PCR Primer, Index 8 (100% over 46bp)
AGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG276110.10700474756785196RNA PCR Primer, Index 8 (100% over 45bp)
CGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC274410.1063459229296087RNA PCR Primer, Index 8 (100% over 44bp)
ACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT272900.10576073163328674RNA PCR Primer, Index 8 (100% over 43bp)
TAAATGGATTTTTGGAGCAGGGAGATGGATGGAATTCTCGGGTGCCAAGG271870.10536156141129227Illumina Small RNA Adapter 2 (100% over 21bp)
TTAAATGGATTTTTGGAGCAGGGATGGAATTCTCGGGTGCCAAGGAACTC271180.1050941561169465RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC264980.1026913839068828RNA PCR Primer, Index 8 (100% over 44bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC264600.1025441172230402No Hit
CCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC263700.10219532770867612RNA PCR Primer, Index 8 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)