FastQCFastQC Report
Tue 4 Jul 2023
SRR6880571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33411351
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG12793643.8291298068132593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA6406901.9175818421709436No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC6396961.9146068053339116No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT4652921.3926165392114793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG2900460.8681061714625068No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT2380090.7123597007496045No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC2007410.6008167703245523No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC1861670.5571968640238463No Hit
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA1807370.5409449022279883No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCA1769360.5295685289708878No Hit
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC1665720.49854913080288193No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC1661190.4971933041558242No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1623380.4858767907948409No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC1560470.46704786047113156No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1512370.45265155545491115No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1353320.4050479730675961Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1217320.3643432437078046Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA1179760.35310155521696807No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC1146600.343176784440713No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1144220.34246445167691664No Hit
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCA1088270.3257186457380906No Hit
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC1061880.3178201324454075No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAGC893150.26731933108601325No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC890170.2664274186338649No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA844990.25290506810095764Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA795320.23803886290021617No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG734680.21988934239743851No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC729820.21843474692178716No Hit
ACTTTTTCTGGAGCAAATCCATGTCTTGAGATCGGAAGAGCACACGTCTG697670.20881226862092464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC622100.18619420687298757No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACAC595000.17808319094908795No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG555410.1662339245126604TruSeq Adapter, Index 5 (100% over 37bp)
TCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC532150.1592722185942137Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG512370.1533520748682087Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG505140.15118813962356684No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA494300.147943733254007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGC477390.14288257903728585No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAGAGATCGGAAGAGCACACGT460610.137860333753041No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC432380.1294111094160784No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG429160.1284473650885892No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCA423630.12679223896094474No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG422790.12654082739725192No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG422310.12639716364657028No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGC421200.12606494122311906No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA420070.12572673281005609Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCCAGATCGGAAGAGCACACGTC417450.12494256817091892No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC407100.12184481854684655No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG406120.12155150505587158No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT401990.1203153982010485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA401230.1200879305958026No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT393810.11786712844984927No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACA392840.11757680795368018No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT382770.11456286218417208No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG381710.11424560473475018TruSeq Adapter, Index 5 (100% over 49bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT381030.11404208108795122No Hit
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC379270.11351531400211862Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT372970.1116297272744224No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCAC372900.11160877631078132No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC372860.11159680433155784No Hit
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG371500.11118975703795994Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAG363420.1087714172348194No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA363200.1087055713490903Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG354800.106191455712162No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC338850.1014176289968041No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC338250.1012380493084521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)