FastQCFastQC Report
Tue 4 Jul 2023
SRR6880572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42091858
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC524343512.457124130752318No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT14984163.5598713651461997No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG14862353.530932276736275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG13290063.1573944775733107Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG11689542.77714991816232No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT4265691.0134240213392338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG3920230.9313511415913263No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC3316620.7879481110099725No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT3240090.7697664474682966Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3199430.7601066220455273No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1984930.4715710102414581No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC1939590.4607993308349562No Hit
TCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1935210.4597587495424887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1928730.4582192594111668No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1787330.4246260642616442No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1735090.4122151129560496No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC1693860.40241986941987684Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1682180.3996449859732968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCAGATCGGAAGAGCACACGTC1616680.3840837817137937No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC1393330.3310212630670758Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1358700.3227940187387309No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA1347060.32002863831765277Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1285010.30528707000769606Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1260340.29942607902934576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG1157700.27504131559124806No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC1146600.2724042260144468No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT1132730.2691090519216329Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT996060.2366395895377201Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG994910.236366377554538No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGAGATCGGAAGAGCACACGTCTGA973760.23134165282036254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG962140.2285810239120354No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC960930.22829355739060034No Hit
ACGGGGGCGGGCGGGCGTCCCGCGCGTGAGATCGGAAGAGCACACGTCTG947340.22506490447630037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG934700.2220619484176726No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA917580.21799465350282235No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT900720.21398912825373495No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT898390.21343557701824423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG884500.21013565141267937No Hit
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGTGAGATCGGAAGAGCACACGT880140.20909982163296284No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA833690.19806443326878084No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA831050.19743723358564974Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA829610.1970951246675782No Hit
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC827400.19657008250859348Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA792160.18819791704134325Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC783310.1860953726490287No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA780470.18542065783838765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT757210.17989464850898243Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC754660.17928883063323076No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC747170.17750938910798378No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG738360.17541634774117124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC733110.17416907564403547No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG730220.17348248205151695TruSeq Adapter, Index 2 (100% over 49bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA727310.17279113694624743Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG712500.16927264175413687Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGAGATCGGAAGAGCACACGT705960.16771889708456206No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT685480.1628533480275449No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCAGATCGGAAGAGCACACGTCTG684070.16251836637859987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA681340.16186978488808929Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT665280.15805432014904167No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG654260.15543623662324432Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC651540.15479003088910925No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA645710.1534049649221947Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT635920.15107909943058345No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC621010.14753684667471795No Hit
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC620880.14750596184183648Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC600100.14256914009355443No Hit
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT597800.14202271612719022No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG581720.1382024998753916Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG559850.1330067206821804Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG558670.13272638142987178No Hit
AAAAAGGATGGGGAAACCTTCCAGCTGATGAGATCGGAAGAGCACACGTC550620.13081389754759698No Hit
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG550250.13072599456170358No Hit
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG521210.1238267980472613No Hit
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACACG516090.12261041078300702No Hit
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC513100.1219000596267335No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC472070.11215233121807072No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT467910.11116401656586412No Hit
TTTTGGGACTGACCCTAGTCTGTGTCCATGAGATCGGAAGAGCACACGTC461920.10974093849694161No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC456040.1083439937481496No Hit
ATGGCTCATCTCATTAACGAAAAGGATGAGATCGGAAGAGCACACGTCTG436170.10362336583003773Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC431980.10262792390870464No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAGATCGGAAGAGCACACGTC427180.10148756084846623No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)