FastQCFastQC Report
Tue 4 Jul 2023
SRR6880575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38518081
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC27259307.07701403919889No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG18329754.758739149024584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA11470452.9779391138411078No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT7842272.035997068493625No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG6677271.7335417099309802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT4380521.1372633023955685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC4015271.042437706073675No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG3525900.9153882821940169No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA3343430.8680157248747673No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT3086730.8013716986575733No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA3057690.7938323822518573Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT2861240.7428303606298559Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC2841550.7377184756426468No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2552970.6627978169525113Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2500350.6491367002421539No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT2424690.6294939771272614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2249920.584120480976194No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG2037400.5289463927343628No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCAGATCGGAAGAGCACACGTC1960610.5090103009025814No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1884720.4893078655709769No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC1820590.47265854184168726No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA1787380.4640366169851504Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCAC1715710.4454297710210433No Hit
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC1662970.43173750011066236Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC1595530.41422883969738783No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1594470.4139536442638459No Hit
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT1367270.3549683588857918Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACA1366620.35479960696899726No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC1318290.34225225290948424Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG1209520.31401356677140796Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1182950.3071155076495114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1175480.30517615869804104No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1131410.2937347787393666No Hit
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC1044570.27118952265560686Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1024680.26602571400169184No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT962920.2499916857228687No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC953580.24756685048769694Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG948280.24619087331998707Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG939920.24402046405167488No Hit
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGTGAGATCGGAAGAGCACACGT936550.2431455502676782No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT923070.23964589513169152No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC890730.2312498382253259No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT875720.2273529670390381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC866830.22504496005395494No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGAGATCGGAAGAGCACACGTCTGA866600.224985247837243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA837040.21731092989809125Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA819090.21265078081122474Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT814580.21147990212700365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG808110.20980017150906352No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA793850.2060980140729233Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT768550.19952967023461007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT724400.1880675208092532No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG723170.18774819025901107No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG720840.18714327954188578No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT613450.15926286670408113Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG610680.15854372392020258No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG603260.15661735588540873No Hit
TGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA601770.15623052456844877Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG595020.15447810081712013Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC587540.1525361556823145No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG573390.14886255626286263No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG567740.1473957126784172No Hit
GCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA564830.1466402233278444No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGAGATCGGAAGAGCACACGT553180.14361566974221796No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC547520.1421462299744372No Hit
TTGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTC514610.1336021906179594No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACGT508580.1320366920667725No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT505150.13114620118276402No Hit
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT490630.127376542979906Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGT474070.12307726337664641No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGCGAGATCGGAAGAGC469820.1219738854591432No Hit
ACCTACTAAACTCAGTCTTTGACCAACTGAGATCGGAAGAGCACACGTCT458320.11898827462354628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC451470.11720988903886465No Hit
TCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT450540.11696844398868159Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA446720.11597670195459633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC436670.11336753770261815No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCAGATCGGAAGAGCACACGTCTG428820.11132953378440633Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT426910.11083366276736371Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA426420.11070644978393394Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACAC422190.10960826423310134No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT421160.10934085734956525No Hit
CCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA418760.10871777334909286No Hit
ACGGGGGCGGGCGGGCGTCCCGCGCGTGAGATCGGAAGAGCACACGTCTG413680.10739891221475961Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC410920.10668236561421636No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG410160.10648505568073342Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA400880.10407579754557346No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC399980.10384214104539632No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT396240.10287116847799349Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG395020.1025544341110867Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC385810.10016334925927385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)