FastQCFastQC Report
Tue 4 Jul 2023
SRR6880578.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880578.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45576120
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG33814997.419453433069774No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC25264825.543433710460653No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG13208422.8981010230796302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG12739272.7951633443127673Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC10737452.3559377147506195No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT5693051.249130026864946No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG4578761.0046401492711534No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA4512870.9901830168956901No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG3486720.7650322142385091No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC3245510.7121075686126858No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3208370.7039585642656725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC2861320.6278112309692006No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2735890.6002902397132533TruSeq Adapter, Index 4 (100% over 49bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2635510.5782655478351382No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT2590310.5683480735086708No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC2253080.49435537733356855No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1832840.4021491956752791No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT1751490.3842999360191258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1728320.3792161333610672Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1649670.36195928920671616No Hit
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1647580.3615007157256914No Hit
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT1568370.3441210002080037No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1472800.32315168557569185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC1291070.2832777340414234No Hit
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC1265110.27758176869816914No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1223300.2684081049461867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1222450.26822160376969345No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT1177800.2584248066750746No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGATCGGAAGAGCACACGTCTGAAC1123630.24653919640373073Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC1109570.24345424753138264No Hit
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC1105540.24257001254165558Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT1064010.23345778447134155No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGTAGATCGGAAGAGCACAC1045770.22945568863694407No Hit
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1033140.2266845005674024Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT972610.21340342266959103No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACA925640.20309758706971984No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC923430.20261268401083726Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGTACACGGAGTCGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG873780.19171882117214015Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC873050.1915586495735047No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA843090.1849850316349878Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG807260.177123458512923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG800970.17574334980687253No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA767640.16843030955684687No Hit
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT756780.16604748276070888Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA725350.15915132749343294Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACA709300.15562974645494174No Hit
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTCT709230.15561438753452467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGGGCGGGCGGGCGTCCCGCGCGTGAGATCGGAAGAGCACACGTCTG691790.1517878222191797Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGTAGATCGGAAGAGCACACGT673830.1478471620664506No Hit
TCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT664160.14572543691740322Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC660770.14498162634291817No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG653060.1432899509655495Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT652950.14326581551917977Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC647610.1420941493045042No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA643730.14124282628709947Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA627630.13771027459116747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT611470.13416455810630656Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA605060.1327581198223982No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC591130.12970169465939618Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCA590180.12949325216802132No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG586760.1287428591990718No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC586230.1286265702301995No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG567260.1244643027971666Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA523170.11479037706588449No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC522120.11455999325962808No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACA521110.11433838597932426No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC517370.11351778080275372No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC497400.10913610022090517Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA496850.10901542298905655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCA496490.10893643425548291No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC462860.10155757006081255No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT462200.10141275738259421Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC462080.10138642780473633No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)