FastQCFastQC Report
Tue 4 Jul 2023
SRR6880579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36286515
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG16385134.515487364934329Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACACG7610142.0972363975983916No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA7415232.043522228574444No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTAGATCGGAAGAGCACACGT5457761.504073896322091No Hit
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACAC5077851.3993766003706887No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC4781541.3177181661010986No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT4537541.2504755554508336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG4331231.1936197234702755No Hit
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTAGATCGGAAGAGCACACG3687991.0163527690658636No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC3400140.9370257794114425No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2789080.7686271332477093No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC2551310.7031014138447851No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTAGATCGGAAGAGCACACGTC2352370.6482766394072288No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATAGATCGGAAGAGCACACGTCTG2146320.5914924593888391Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGC2118810.5839111306224917No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC1852200.5104375551082819No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTAGATCGGAAGAGCACACG1699950.4684798195693359No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1643060.4528018190779688No Hit
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTAGATCGGAAGAGCACACGT1614530.44493939415234557No Hit
GCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC1560020.4299172846992884No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC1373870.37861723563147354No Hit
TTCCGGCCGTGCCGGTGGTCCCGGCGGATAGATCGGAAGAGCACACGTCT1367580.37688380931594007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCAC1315440.3625148350564941No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC1276970.3519131005002823No Hit
ACCCGCGCGTCGTTCCCCTCTTCCTAGATCGGAAGAGCACACGTCTGAAC1274000.35109461462474423Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCCGCGCGTCGTTCCCCTCTTCCTAGATCGGAAGAGCACACGTCTGAA1268570.3495981909533059Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1241250.3420692232362353Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC1212800.33422884506820233No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1185640.3267439708663122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1174550.32368773909536364No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACA1146060.3158363375485356No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1145310.31562964919612696TruSeq Adapter, Index 7 (100% over 49bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT1140680.31435369310059125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG1125040.3100435519916972Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCA1084050.2987473445713924No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1070380.29498010486815834No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT1034270.28502874966085884No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA925660.2550975203873946No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC887170.24449027414178517No Hit
TCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA880200.24256945038673458No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG863340.23792309622458924Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGT808790.22288996339273692No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT761820.20994576084256092No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT760870.20968395559617672No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT710680.1958523710529931No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT667990.18408767003389553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT664900.1832361140219721No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG657120.18109206684632018No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA646890.17827283771946686No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC636760.17548116703960134No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG624190.17201707025323318No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCA615040.16949547235384826No Hit
TGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACACG614100.16923642295216282No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG610850.16834077342505888No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG610240.16817266689843322No Hit
TCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGA604390.16656049774964612Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG598680.16498691042664196No Hit
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG586270.1615669071554543Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG585140.161255496704492No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACAC582780.1606051173555796No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT581590.16027717183642465Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA568260.1566036308529491Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCA543640.1498187412045494No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC522030.1438633608104829No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTC521340.14367320752626697No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC494970.13640604505558057Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC481550.13270770146981598No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCA481000.132556130011383No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA475920.13115616090440207No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC463410.12770859918622662No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA460830.12699759125394103Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC459840.12672476262876167No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC451610.12445670244166462No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA443930.12234021371300055No Hit
CCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA436280.1202319925184328Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC434440.11972491709385705No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG413320.11390457309003084No Hit
GCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACACG410290.11306955214630007No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCAC402420.11090070236835915No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG399440.11007946064812231No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA386490.10651064176320046Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG385280.10617718455464792No Hit
GGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACACGT384590.10598703127043199No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG378010.10417368545863387No Hit
TTCGGTCACGCGTCTCCCGACGAAGCCGAGCGCAGATCGGAAGAGCACAC368570.10157216806298429No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA365910.10083911337310844Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)