FastQCFastQC Report
Tue 4 Jul 2023
SRR6880580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43148087
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACACG12024892.786888327169638No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTAGATCGGAAGAGCACACGT9486002.198475218611662No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG9263572.1469248451269696Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC9199402.13205280688342No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCAC7637751.7701248261597322No Hit
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACAC7632271.768854781441411No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGC7383371.7111697211512529No Hit
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTAGATCGGAAGAGCACACG6160671.4277967873755328No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3660030.8482484982474426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCA3589530.8319094192982414No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTAGATCGGAAGAGCACACGTC3521980.8162540323050707No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3022320.700452838152477No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3013890.6984991014781258No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA2952430.6842551327941839No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT2852270.6610420526870635No Hit
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTAGATCGGAAGAGCACACGT2336010.5413936427818921No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG2183360.5060154810571323No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACA2097240.4860563111407465No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC1951930.45237926770658454No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC1825610.42310334638937763No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT1819750.4217452328767206No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTC1758880.40763800258398475No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC1590800.3686837842892085No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT1479670.34292829714559536No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1455750.33738459830212175No Hit
GCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC1215800.28177378987856405No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACAC1214970.2815814290909351No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1160930.26905711949639854No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1103000.25563126355984217No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1061880.24610129297273367No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG1053060.24405716990419526No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1003560.2325850506419902Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC985890.22848985170536065No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC946580.21937936669127414No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC929400.21539773014734118No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG849270.19682680254167467No Hit
CCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCA827410.19176052926749684No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA814160.18868970946498742No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT802220.18592249524295248No Hit
TGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTAGATCGGAAGAG800340.1854867864709738No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA797180.18475442491807342No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC788200.18267322025191987No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG767560.177889694159558No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC750970.1740447960068311No Hit
CCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCACA730130.1692149179174502No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG722210.1673793788354974No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACA718540.16652881969019853No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT694300.1609109576514945No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC682540.15818546022677668No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG676430.15676940671784592Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCAC669970.15527223721413186No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC656240.15209017261877683No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT649480.15052347511953426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACA632540.14659746097202409No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC618280.14329256358456866No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC617690.14315582519336256No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC613880.14227281965015043No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC604810.1401707565853383No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT604260.14004328859353604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA594500.13778131113900832No Hit
CCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA583670.1352713505004289Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA583490.13522963370311178No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC571640.13248327787973543No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCA569600.13201048751014152No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCA567720.13157477873816284No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG563800.13066627959659022Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA556100.1288817277113583Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA531170.12310395128293868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGGGATCGGAAGAGC525540.12179914256685355No Hit
CCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACACGT525430.12177364896849309No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG523600.12134952819576914Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCA519810.1204711578522589No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC513750.11906669234258288No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC476110.11034324650360512Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC473590.1097592113411656No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA470870.10912882418170707Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC467340.10831071143432153No Hit
TGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTAGATCGGAAGAGC462080.10709165391272156No Hit
GCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACAGATCGGAAGAGCA453990.10521671563330258No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC453190.10503130764522656No Hit
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC450380.10438006208710944No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG447690.10375662772720376No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG432410.10021533515495136TruSeq Adapter, Index 8 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)