FastQCFastQC Report
Tue 4 Jul 2023
SRR6880581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39389251
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG18305334.647290703750625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG11210682.8461267262990098No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC10590572.6886954514570482No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG7538801.9139231665004242Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT4230521.0740290542716846No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC3570120.9063691005447146No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3188240.809418792959531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT2942440.7470159815935571No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA2712460.6886294943765242Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC2642590.6708911525126487No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2385540.6056322320015681No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2217780.5630419324297382TruSeq Adapter, Index 1 (100% over 49bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG2126440.539852864935157No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC2046410.5195351391677897No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC1893560.4807301362496078No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1643460.417235656499282No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1563690.39698393858771264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1503290.3816498059330958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC1486040.37727043857726567No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1407150.3572421318699358No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA1333790.3386177614801561Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1230780.3124659567657176Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1212370.30779209282248093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG1166460.2961366287467614No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1121130.2846284129647451No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1110430.2819119358222882Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTC1103310.28010433607889623No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1087300.27603977541994895No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACA1071800.27210469170891316No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA977370.24813114623580937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA902010.22899902310912182No Hit
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG885900.22490907481332914Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC884050.22443940353168937No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA875270.22221036901666397Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA817790.20761755535793255No Hit
TCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTGAACTC805240.20443140693383582Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC800870.2033219672036922No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG774240.19656123951176424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGTAGATCGGAAGAGCACAC752740.19110289759000496No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC733310.18617007975094527No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT728260.18488800409025294No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGT713660.1811814091108257No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA712300.18083613724972836Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT692170.1757256059527509No Hit
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG690660.1753422526363855No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG688690.17484211619053128No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC656020.16654797523314163Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT640270.16254942242999237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA622640.15807358205414973Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG592740.1504826786373775No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT590680.1499596933183624No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTAGATCGGAAGAGCACACGTCTG574000.145725035492551Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCAGATCGGAAGAGCACACGTC573990.14572249672886645No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT570800.14491263111349845Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT559710.14209714218734446Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTCT555940.14114002827827318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACG546150.1386545786311093No Hit
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG535350.13591271385180692No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT528880.13427013374791005No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT524770.13322670187356445No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC509950.12946425409307732Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG503190.12774804984232882No Hit
AAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGAAGAGCACACGTCT492410.12501126259039555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC467570.11870497359800013Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT449350.11407934616476968No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG439780.11164974931866564Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT437540.11108106625332885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA435580.11058346857115917Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACT435440.11054792587957563Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC432090.10969744004525499No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC416600.10576489509790375No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA398080.10106310475413711Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)