FastQCFastQC Report
Tue 4 Jul 2023
SRR6880583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41014197
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA14597333.5590919895371838Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT12989833.167154534318933Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG10220962.4920541538336103Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG5950191.4507635002582155No Hit
AGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG5842241.4244433458004797Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG5184861.264162260692316Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG5081091.2388612655271538No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA4901981.1951910212944068Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG4893661.1931624554297626Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT4628511.1285141093948519No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCT4309691.0507800506249092Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA4120191.0045765372414825No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCTG3878640.9456822963033995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCC3746530.9134714986617927Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2813270.6859259002437619TruSeq Adapter, Index 5 (100% over 49bp)
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2537570.6187052741761591Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT2295140.5595964733870079No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCTG2272190.5540008499983554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAGATCGGAAGAGCACACGTC1882730.45904348681994184No Hit
AGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1871220.4562371414951754Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1851630.45146074662878316Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1785220.4352687924135148Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGTGGGGGGCCCAAGTCCTTAGATCGGAAGAGCACACGTCTGAACTCCA1746300.4257793953640004Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTC1690890.412269439287084No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1661090.40500366251227593Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTGGGGGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA1630860.3976330439920597TruSeq Adapter, Index 5 (100% over 36bp)
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTC1583340.38604681203437924No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC1572400.3833794429767819No Hit
GCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGT1568060.38232127280219574No Hit
AAGTGGGAGGCCCCCGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA1422050.34672140478576235Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1321980.32232253626713697Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1255970.30622810925689953Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT1223780.2983796074320314Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1215250.2962998397847457No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTG1194390.2912137960423802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1177700.2871444734124625Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1171950.2857425198401422No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1161530.2832019361490851No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1157320.28217546231613405No Hit
TCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA1130880.2757289140635863TruSeq Adapter, Index 5 (100% over 40bp)
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT1103320.2690092896369518No Hit
TAGGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTC1101170.2684850809099103Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1018880.24842129665491194No Hit
TGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT989080.24115551988010395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAACT952550.23224884788064973Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGACGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA951230.23192700810404748Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA913010.22260828366333735Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG876600.21373086982539238No Hit
TAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCACACGTCTGAACTCCA848260.20682106734894748Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC843730.20571657175197164No Hit
AAGTGGGAGGCCCCCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG832500.20297849547072688Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGT818920.19966744685992513No Hit
TTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACT811420.19783881176559426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACACGTC809460.19736092846094244No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTG804880.1962442419633377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA798460.19467893032259048Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC778760.18987571547481472No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACAC724800.17671929551613555No Hit
CCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACT722760.17622190677047755Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC702610.17130897381704194Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG697470.17005574923239383Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA688610.16789552164095764Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA669780.16330442846412427No Hit
AAGTCCTTCTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA658450.16054197038162177TruSeq Adapter, Index 5 (100% over 36bp)
TGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG652540.15910100592728904Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC647170.15779170319974814Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTGGGGGGCCCAAGTCCTTCAGATCGGAAGAGCACACGTCTGAACTCC636640.1552242995273076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTGGGGGGCCCAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA616020.15019677210796056Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC611390.14906789470972698No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC605340.1475927957336334No Hit
TCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAAC600550.14642490745338743Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC587990.14336255321541466TruSeq Adapter, Index 5 (100% over 35bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG585510.14275788454422259Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGGAGGCCCCCGGCGCCCGGCAGATCGGAAGAGCACACGTCTGAAC578370.1410170239344196Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC577530.14081221680385453TruSeq Adapter, Index 5 (100% over 35bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCA570070.13899333443002676No Hit
GCCGCGGCTGGACGAGGCGCCGCCGCCCTAGATCGGAAGAGCACACGTCT569020.13873732551682044Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGCCCGGTGGGGGGCGGGGCGGACAGATCGGAAGAGCACACGTCTG564110.13754017907506516Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA553660.1349922808436308Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCA552020.13459241930300378Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACGT539640.13157395230729496No Hit
CTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACT537020.13093514911434204Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC533720.13013054967283647No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA525170.12804590566529928Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCCCGCCTCCCCTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT513760.12526394214179057Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC512880.1250493822907224No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTC507960.12384979766884135No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC507470.1237303268426784No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACAC501980.1223917659536282No Hit
CGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGTC498480.12153840290960714No Hit
AAGTGGGCGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT497670.1213409103194194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATGACGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA497650.12133603395916785Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGTGGGAGGCCCCCGGCGCCCAGATCGGAAGAGCACACGTCTGAACTCC497590.1213214048784132Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC494310.12052168179715915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCCTCCCGGCTCCGGCCGGGGGTAGATCGGAAGAGCACACGTCTGAAC492070.11997552944898568Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTCTGA488340.1190660882620718Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTG472510.11520644912297077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACG469600.11449693870637039No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT464960.11336562312801102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA460150.11219285848751348Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA455470.11105179018865102Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGCCCGGTGGGGGGCGGGGCGGACAGATCGGAAGAGCACACGTCTGA453210.11050076148022597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA452420.11030814525028979TruSeq Adapter, Index 5 (100% over 36bp)
TTGGGGAAGGTGGCTCGGGGGGGGCAGATCGGAAGAGCACACGTCTGAAC448540.10936213136148928Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG438860.10700197299973958TruSeq Adapter, Index 5 (100% over 37bp)
GGGGGCCCGGTGGGGGGCGGGGCGGACAGATCGGAAGAGCACACGTCTGA435040.10607058819169371Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCAGATCGGAAGAGCACACGTCTGAA427240.1041688076935896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACT424870.10359095900378106Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGGGTCGGCGGCGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTC422600.10303749211523024Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA419710.10233285805888143Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG416810.10162578582240682TruSeq Adapter, Index 5 (100% over 37bp)
AGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG412050.10046521208253816Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)