FastQCFastQC Report
Tue 4 Jul 2023
SRR6880584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45561965
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG472137710.362540333806937No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT20326224.461225498066205No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC11692302.566241381380281No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG11356162.492464932098517No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT10746362.3586252261069074No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG10567202.319302953680773Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA10405552.2838237990832924No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC9466102.0776320775453825No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA8277671.8167938981560605No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA8169931.7931469812594782No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG5972001.3107424142044795No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG5386121.1821527012717734Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA5043661.1069891300781254No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG4607721.0113084455422412No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC4420500.9702171537158242No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG4322980.9488133358602948No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC4193340.9203597781614555No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC4144880.9097237136282423No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC4134550.9074564716425202No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC4043460.887463918643544No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACGT3571210.7838138675537809No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTC3125120.6859054476689055No Hit
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC3069970.6738010531371945No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC2644910.5805083253103768No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACGT2577150.5656362713943528No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT2328810.5111302815846508No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACA2306090.5061436661039531No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC2179790.4784231759977868No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1960500.4302931183938182No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACAC1813030.3979262088454701No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC1727100.3790661794327791No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACA1691420.37123508610745826No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT1664760.36538371424498484No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC1655600.36337326539801346No Hit
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA1589790.3489292000465739Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC1531920.33622781633759646No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC1454970.319338729135146No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1436060.315188337465252Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA1369390.30055551818276494Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1343740.29492582244861476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGCA1283290.28165817694649475No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG1271560.2790836611195325No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC1227320.26937380773634323No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC1135990.24932857922172585No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1131980.24844845914788793No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCA1107070.24298117958696472No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCAGATCGGAAGAGCACAC1099940.24141627780979155No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACG1099110.2412341083181992No Hit
CGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACACG1052730.23105456492054283No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGC1051840.23085922655004015No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1017200.22325639379249776No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG930330.20419005194354545Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG925540.20313873644387373No Hit
CCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA865030.1898579220628434Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCAC848650.18626281812033346No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT829840.1821343745819567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA818940.17974202824658683No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG779120.1710022822764558No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACG771630.16935836722582093No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG732130.1606888552765448No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT726250.1593983051433361No Hit
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA718790.15776097453215637Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC678860.1489970856173565No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC660530.14497399311026204No Hit
ACCCCCCTCCGTCGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG652130.14313035006282104Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA646660.14192978726883268No Hit
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACACG630690.1384246706655431No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACAC598370.13133103455919867No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT564730.12394768311682783No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA562280.12340995389465753Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG546810.12001457794895368No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACAC545780.119788512194327No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC531210.11659066943227757No Hit
ACCCCCCTCCGTCGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT505540.11095658407182395Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG504830.11080075233805214No Hit
TCGTGGGGGGCCCACGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA484030.10623554098248397No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG464280.10190078500784591No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG463070.10163521261648834Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)