FastQCFastQC Report
Tue 4 Jul 2023
SRR6880585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39796581
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG25832746.491195814032366No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC20049655.038033292357452No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC12689333.1885477800216053No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT12133713.048932771385562No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT12116533.044615817625137No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA11541702.900173761158025No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC6255261.5718083922837491No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTC5548401.394190118995398No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG4769291.1984170197937356No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA4229621.0628098931413228Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT3944780.991235905416096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC3908360.982084365488583No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG3805900.9563384352037678Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC3788210.9518933297310138No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT3737440.93913595240757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA3265970.820665976305854No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG3243440.8150046859553086No Hit
ACCCCCCTCCGTCGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT3076300.7730061032127358Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG2832330.7117018419245613Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGACGCAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA2595930.6522997540919407No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2519010.6329714605382809No Hit
ACCGCCCGTCCCCGCCCCTTAGATCGGAAGAGCACACGTCTGAACTCCAG2492430.6262924948251208Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC2208120.5548516843695693No Hit
GGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT2148910.5399735218460098Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2069180.5199391374852026No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1988260.4996057324622937Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA1932960.4857100663999252No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT1862680.4680502578852188No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACG1842530.4629870088588766No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1794020.45079751951555835No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1472770.37007450464149166TruSeq Adapter, Index 7 (100% over 49bp)
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1456780.3660565715431685No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACG1351060.33949147541091534No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1307470.32853827317477347No Hit
TGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1248340.31368021288059894Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1240300.31165993882740833No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCT1234870.3102955000079027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC1192930.2997569062528261No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACAC1164900.2927135876320631No Hit
TCTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA1141420.28681358330757106Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACAC1116380.28052158551007184No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACACGTC1051060.2641081152172344No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1044490.2624572196289927No Hit
CCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACT985690.2476820810310313Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAAC985530.2476418765722613Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC949030.23847023441536347Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACT934010.23469604084833318Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG930390.23378641496866276Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA903300.22697929754317336Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC887800.2230844905998332No Hit
TCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA874100.2196419838176551Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA866100.21763176087915692Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT865980.21760160753507946Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT846130.2126137418689309Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCAGATCGGAAGAGCACACGTC837030.21032711327638925No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC765430.19233561797683071No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT750610.1886116799832629Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGCAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA737000.1851917882091429No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG717960.18040745761551727Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACGT715430.1797717246112172No Hit
TAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC714950.17965111123490735Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC713590.17930937333536265No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG703100.17667346850725696No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA690010.17338424122413934Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG669240.16816519992006349No Hit
ACCCCCCGTCCTTTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC665900.16732593184324052Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC665300.16717516512285313Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAACT663920.16682840166596222Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC651260.16364722386578887No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT639590.16071481115425468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGT609660.15319406458559845No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC579570.14563311355817224No Hit
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA574060.14424857250928164Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTG560170.14075832293231422Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGA548590.13784852522883811Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCAGATCGGAAGAGCACACGT548040.13771032240181638No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT545450.1370595127254776No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCAGATCGGAAGAGCACAC543810.13664741702308547No Hit
TCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA533420.134036639981711Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG533170.1339738205148829No Hit
CGTTTTTTCCCTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT530860.13339336864139156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCAGATCGGAAGAGC525100.13194600812567292No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC487920.12260349701900271No Hit
TTGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA487220.12242760251188413Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC482860.12133203101040264No Hit
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA480470.12073147690752631Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCA469150.11788701144955141No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG463690.1165150342940264Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA460210.1156405873157797Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCGAGTGTTACAGCCCTCCGGCCAGATCGGAAGAGCACACGTCTGAA451210.11337908650996928Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG438170.11010242312021729Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT425210.10684586195985027No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA401860.10097852375810876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)