FastQCFastQC Report
Tue 4 Jul 2023
SRR6880586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37188761
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC24679766.636349084068706No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG21853675.87641787797125No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA9312742.5041813035933087No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT8740502.350306857493854No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTC7018361.887226089624228No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC6918151.8602797764625714No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT6236871.6770846439331497No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT6175301.6605285666817455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC6038591.6237674602818848No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA4990721.3419968468430556Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG4467581.2013253143873226No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG3581860.9631565837861606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACG3024550.8132967914687989No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT2794350.7513963694568905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC2543390.6839136157292253No Hit
ACCGCCCGTCCCCGCCCCTTAGATCGGAAGAGCACACGTCTGAACTCCAG2422070.6512908563961031Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA2306660.6202572868722355No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG2288320.6153256893930938No Hit
GGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT2214110.5953707357983774Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGACGCAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA2167870.5829368717070192No Hit
ACCCCCCTCCGTCGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT2027170.5451028605120778Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGA2013440.5414108848638436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAAC1875010.5041872731387852Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC1860840.5003769821748028No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1606850.43207946615914417No Hit
TGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1588170.4270564432087426Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAACT1518500.4083222885537918Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT1392100.37433352512066753No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1333940.3586943915663122No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1238560.33304685789343724No Hit
CTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACT1229850.3307047524385123Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1226890.32990881304166064No Hit
TAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC1142670.3072621860136722Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1119830.30112054553256024TruSeq Adapter, Index 8 (100% over 49bp)
ACCCCGAGTGTTACAGCCCTCCGGCAGATCGGAAGAGCACACGTCTGAAC1021640.27471740722956595Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACGT1013930.2726442002195233No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCAGATCGGAAGAGCACACGTCTGAA1001700.2693555722386126Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT980660.26369794895828874No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC973300.2617188564039549No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG970950.2610869450584815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA952390.2560961899214658Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG932780.2508230914173236Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA892680.2400402637775429Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACT885340.23806654919210674Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT862010.23179314847300236Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC856680.23035991976177966No Hit
TCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA846010.22749077335488538Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCGCCGTCGGGTCCCGGGGGGACAGATCGGAAGAGCACACGTCTGAA845070.22723800881669598Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT840260.22594460729681207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC817290.21976801001786536Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCA779690.20965742849029037No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA758830.20404820693004536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCAGATCGGAAGAGCACACGTC754470.20287580971035846No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACG747410.20097738668948933No Hit
TGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAAC710390.19102276625994613Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACACGTC708490.19051185921466973No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC696150.18719365240482197No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACG694360.18671232418848266No Hit
GGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG679530.18272456025087797Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACAC658920.17718256330185347No Hit
ACCCCCCGTCCTTTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC626190.1683815172008554Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC623600.1676850702286102Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA616440.16575975736325285Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA603790.16235819203549157No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCT574340.15443913283370747Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTCGCGCCGTCGGGTCCCGGGGGGACAGATCGGAAGAGCACACGTCTGA561420.150964964925828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGT552160.14847496532621776No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG546740.14701753575495563No Hit
CCGACGCAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA541010.14547674766577998No Hit
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGA536030.14413763341026609Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA534470.14371815183624967Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCAGATCGGAAGAGC516400.13885915693722628No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT508740.13679939484942777Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC504130.1355597730185203No Hit
ACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGAAC495140.1331423759990283Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCAGATCGGAAGAGCACACGT494130.13287078856969717No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC477090.12828875906890255No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCAGATCGGAAGAGCACAC471840.12687704223327043No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACAC465440.12515609218602364No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG464140.12480652420767663No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA459660.12360185917460387Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC457480.12301566056476042No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG450900.12124630879743478No Hit
TAACCTCTTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGA448140.12050414908955961Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGGACGAGGCGCCGCCGCCCAGATCGGAAGAGCACACGTCTGAACTCC420310.11302070536848485Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT417540.11227585667616086Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGAGATCGGAAGAGCACAC412260.11085607288718223No Hit
TCTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA406980.10943628909820362Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC400890.10779869756887034Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGA390740.10506937835331487Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTTTTTCCCTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT387990.10432990762988852Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTG385960.10378404378677741Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA384930.10350707838854861No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT383900.10323011299031984Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT381370.10254979992476759Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCCGCCTCCCCTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT380990.10244761851571232Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGGACGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG380360.10227821249543646Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC374420.10068095573283552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)