FastQCFastQC Report
Mon 10 Jul 2023
SRR6880587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40095204
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTAGATCGGAAGAGCACACG12685563.1638596975338995No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC9410812.347116128901601No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG9102282.2701667760563082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC8803892.195746403983878No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA8775462.1886557803771245No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG8450992.1077308897094027Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAACT6113611.52477338686193Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC5701061.4218807815518284No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT4627681.1541729529546727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG3905030.973939426770344No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG2962040.7387516970857662Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG2318150.5781614180089968No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT2303430.5744901559797526No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC2131520.5316147038433824No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2071940.5167550712549063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1973160.49211870826246457Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACACGTCTGAAC1896500.47299921456940336Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT1715070.4277494136206415No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC1660400.41411436639653953No Hit
TCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGA1526160.38063405289071484Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA1440230.3592025619822261Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA1399320.34899934665502635No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGAGATCGGAAGAGCACACGTCTGA1363480.34006062171425794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1345880.3356710692879876Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG1328410.33131393969213874No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1327710.33113935522063936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC1288420.3213401782417668Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1186110.29582341070019247No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTC1170370.29189775415533487No Hit
GCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTAGATCGGAAGAGCACACG1138000.28382446938042766No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA1095620.2732546266630792Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGAGATCGGAAGAGCACACGT1077220.26866554912652396No Hit
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA1076410.2684635299523604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT1054510.26300153005830823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1053250.26268727800960934No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1041520.2597617410800554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC1038940.2591182725993862No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT988930.24664545914269445No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG984360.24550567195019135No Hit
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC967220.241230846462335Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA945120.2357189652907116Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT893320.22279971439975715Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA864700.2156617035793109Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGC858180.21403557393048803No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC848430.21160386164888947No Hit
ACCCGCGCGTCGTTCCCCTCTTCCTAGATCGGAAGAGCACACGTCTGAAC823530.2053936425912685Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT799610.1994278417937467No Hit
ACGGGGGCGGGCGGGCGTCCCGCGCGTGAGATCGGAAGAGCACACGTCTG781760.1949759377705124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT778400.19413793230731535Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCCCGACCCCTCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGA759490.18942165751295342Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAAC757930.18903258354789765Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG742890.18528151147453945No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA736100.1835880421009954No Hit
ATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAA730310.18214397911530766Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTAGATCGGAAGAGCACACGTCTG719000.17932319286865334Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG717270.17889171981766197No Hit
TTGGGGAAGGTGGCTCGGGGGGGAGATCGGAAGAGCACACGTCTGAACTC689040.17185097748847966Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTC674140.16813482230942134No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGAGATCGGAAGAGCACACGT663910.16558339496165178No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC648990.1618622516548363No Hit
TCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT629870.15709360151902457Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC624250.15569193761927236Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACACG623930.15561212757515835No Hit
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC614010.15313801620762424No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGAGATCGGAAGAGCACACGT608350.15172637605235778No Hit
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC607850.15160167285842965TruSeq Adapter, Index 5 (100% over 35bp)
TCTCCCGACCCCTCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAA606570.15128243268197364Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG601940.15012768110619915Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG600410.14974608933277905No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC598150.1491824308962239No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTAGATCGGAAGAGCACACGTCT577650.14406959994517052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG570350.14224893331381977No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC558890.139390736108987No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG556930.13890189958878874No Hit
TACCCGCGCGTCGTTCCCCTCTTCCTAGATCGGAAGAGCACACGTCTGAA537580.13407588598377002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC536550.13381899740427808No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG529560.1320756467531628No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG506430.12630687700204743Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC501280.1250224341045877No Hit
GGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA495510.12358335924665703Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC490230.12226649351877596No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCAGATCGGAAGAGCACACGTCTG489860.12217421315526915Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA477990.1192137593314153No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC472680.11788941141189853No Hit
TCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCACA466470.11634059774331114No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCAGATCGGAAGAGCACACGTC462110.11525318589225783No Hit
TCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTGAA456590.11387646263129125Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT453830.11318810100080798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACACG451440.11259201973383151No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTAGATCGGAAGAGCACACGTC450940.11246731653990337No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA448280.11180389554820572No Hit
GCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCAGATCGGAAGAGCACA448260.11179890742044858No Hit
GCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC447710.11166173390712764No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTCT444640.11089605629640892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA442390.11033489192373233Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA442180.11028251658228251Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA437710.10916767002856502Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCGTGCATTTATCAGATCAAAAGATCGGAAGAGCACACGTCTGAACTC430710.10742182531357117Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT429470.1071125613926294No Hit
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGTGAGATCGGAAGAGCACACGT424980.10599272671115477No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC422760.10543904453011388Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG416340.10383785552007666Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG414230.10331160804169996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)