FastQCFastQC Report
Mon 10 Jul 2023
SRR6880588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40609372
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG14023253.4532053339805397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA9524102.345296056289666No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC8400212.0685397449633056No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC7747741.9078699370184793No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTAGATCGGAAGAGCACACG6334831.5599428624505691No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG6009621.4798603632678682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG5106681.2575126746604208No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC4312061.061838631732596No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT4224871.040368218449672Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA3276490.8068309945792808No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2971400.7317030167321966Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGC2838800.6990504556435888No Hit
ATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAACT2808580.6916088236971505Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2645170.6513693440026603No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2432870.5990907714603417Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT2000970.4927360117758039No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1981440.48792677709963106No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG1764880.43459918562641153No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1666370.41034123847076487No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG1545410.380555010798985Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC1530940.3769917939139763No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG1517350.37364527577525697No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC1444700.3557553167776148No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC1398520.3443835575689277No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT1375030.3385991785344526No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC1370080.3373802480865747Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGA1309110.32236647244877364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1305960.3215907894364877Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA1203490.2963576979225387No Hit
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC1196610.2946635077242761No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT1194020.29402572391417425No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1188400.2926418069208261No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG1153300.28399848192678284No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGAGATCGGAAGAGCACACGTCTGA1044180.2571278373868968Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA1026200.2527002880024838Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGAGATCGGAAGAGCACACGT1016990.2504323386237049No Hit
TCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1007880.24818901410245892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT997810.2457092909488972Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTC994730.2449508453368843No Hit
ACCCGCGCGTCGTTCCCCTCTTCCTAGATCGGAAGAGCACACGTCTGAAC979200.24112660496202698Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT972310.2394299522780111Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACACGTCTGAAC967960.2383587709753305Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC965170.23767173745016298Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCCGCGCGTCGTTCCCCTCTTCCTAGATCGGAAGAGCACACGTCTGAA951340.23426611965336477Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA941030.23172729684172413Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC924690.22770359512085045No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC851620.20971021172157006No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC846940.20855776838903098No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT821410.202271042260885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG820740.20210605571541465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG798620.1966590372291401No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA793730.19545488169578196No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA792130.19506088397525576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT777050.19134745545929643Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACACG746610.1838516488262857No Hit
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC736660.1814014755017635Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG699480.1722459534710362No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTAGATCGGAAGAGCACACGTCT690570.170051878664856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT687380.1692663457095569Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG686480.1690447219917609Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC678090.16697869644475172Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA662900.1632381805855062No Hit
TCCTCCCGACCCCTCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGA647840.15952967704105347Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC640050.15761140063924162TruSeq Adapter, Index 6 (100% over 35bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT639520.1574808888943173No Hit
TCTCCCGACCCCTCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAA633640.15603294727138356Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT620970.1529129778219668No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC618310.15225795661159203No Hit
GCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTAGATCGGAAGAGCACACG617600.1520831201231085No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC616710.15186395889106583No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTAGATCGGAAGAGCACACGTCTG616410.1517900843184672Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG616380.1517826968612073No Hit
TTGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT600840.1479559940005967Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC598480.14737484736282058No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC593710.1462002416585019No Hit
TTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC590640.14544425853224227Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACACG588380.14488773675199904No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC583220.14361709410330206No Hit
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG576610.14198939102037825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTC570860.14057346171223725No Hit
TTGGGGAAGGTGGCTCGGGGGGGAGATCGGAAGAGCACACGTCTGAACTC565190.13917723229012258Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG559330.13773421563869542Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTCT541920.13344702794221985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC538440.13259008290007537No Hit
TTGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTC533710.13142532713876984No Hit
CCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA531400.13085649292976015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA529160.1303048961210235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC524500.12915737775999098No Hit
GCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC522310.12861809338002075No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGAGATCGGAAGAGCACACGT519930.12803202177073805No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA516430.127170151757087Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGCA513040.12633536908672216No Hit
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC510220.12564094810429474Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT502700.12378915881782165No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT499840.1230848878923811No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG493510.12152613441054937No Hit
GGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA492040.12116414900481592Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCAGATCGGAAGAGCACA485090.1194527214062803No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAGATCGGAAGAGCACACGTC478100.11773144386473151No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA476800.11741132071680399Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAAC476740.11739654580228426Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGGGGCGGGCGGGCGTCCCGCGCGTGAGATCGGAAGAGCACACGTCTG474020.11672674967738975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA451600.11120585661851654Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG446670.10999185114214521No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG445320.10965941556545125TruSeq Adapter, Index 6 (100% over 49bp)
ACTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA443010.10909058135644156No Hit
GCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCAAGATCGGAAGAGCAC441250.10865718386386276No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC437880.10782732616500448No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCAGATCGGAAGAGCACACGTCTG432080.10639908442809703Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT430650.10604694896537677No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT429890.10585980004812683No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC424180.10445372068299899No Hit
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGTGAGATCGGAAGAGCACACGT421050.10368296264221964No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG414620.10209958430285503No Hit
TCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTGAA413290.10177207369766762Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG406700.10014929558625038No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)