FastQCFastQC Report
Mon 10 Jul 2023
SRR6880590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39547285
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG11931353.0169833403228563Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAACT8339122.1086453848854605Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG4282671.082923897304202TruSeq Adapter, Index 2 (100% over 49bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA4011531.0143629328789574Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT3375780.8536060060760177Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA3236890.8184860224918095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG3095170.782650439846882Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACACGTCTGAAC2919370.7381973250502532Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2624790.6637092786521249Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC2464080.6230718493064694Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2401180.6071668383809407Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2124740.5372657061034658No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2076250.5250044345648507No Hit
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA2016090.5097922651327391Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA1807470.45704022412663725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1780120.45012445228540976No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1743060.44075339179415224No Hit
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1726690.4366140431637721Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG1687010.42658048460216674TruSeq Adapter, Index 2 (100% over 35bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1679700.42473206441352424Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC1652190.4177758346748709Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1475480.3730926155866326TruSeq Adapter, Index 2 (100% over 37bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA1465450.370556411141751Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1448420.36625017368448937Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1438540.3637518985184444No Hit
TTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC1406590.35567296212622435Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCGCGCGTCGTTCCCCTCTTCCTAGATCGGAAGAGCACACGTCTGAAC1404930.35525321144043137Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT1380700.3491263685990075Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1371730.346858197724572TruSeq Adapter, Index 2 (100% over 37bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1368350.34600352464145134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA1332410.33691566943217466Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA1298420.32832089484777527Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA1275980.3226466747338029Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT1262090.3191344235135231Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCGTCTCTCGGGGTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCC1228620.31067113709575767Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGAGATCGGAAGAGCACAC1226640.310170470615113No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1184220.2994440705600903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTAGCGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT1128960.28547092423664483Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAA1119100.2829777063077782Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG1116090.28221659211245476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1114020.2816931680645081Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA1089120.27539690777761355Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC1080910.2733209119159507Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1077250.2723954375123349No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA1012890.2561212482727955Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1006240.25443971691103445Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC987800.24977694423270777No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGAGATCGGAAGAGCACACGT804290.20337426450387175No Hit
TTTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT777540.1966102097779911Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAGCGAATGATTAGAGGTCTTGGGGAGATCGGAAGAGCACACGTCTGA760460.1922913292277839Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC753820.19061232648461204No Hit
TATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAA743370.1879699200589876Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC737070.1863768903478456No Hit
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA721220.1823690298840995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT718380.18165090220479105Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCCGCGCGTCGTTCCCCTCTTCCTAGATCGGAAGAGCACACGTCTGAA712670.18020706099040681Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT706990.1787708056317899No Hit
TCCCCCGCCTCCCCTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT704770.1782094523050065Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGA690950.1747149014148506Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG677110.17121529328751645Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA676340.17102058965615466Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG660210.16694192787191334Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAAC656130.1659102514875547Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG654730.1655562448850787Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGAGATCGGAAGAGCACACGT651420.16471927213208187No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT649050.16411998952646178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG647210.16365472370606476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA647010.1636041513342825No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACACG637330.16115644854001987No Hit
ATGGGCGAGTGCCGTTCCGAAGGGACGGGCGAGATCGGAAGAGCACACGT626030.15829910953432075No Hit
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA624040.157795914435087Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA619180.15656700580077748Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA612370.15484501654159066Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCA605960.1532241720259684Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCCGTGGATCGCCTCAGCTGCGGCGGGAGATCGGAAGAGCACACGTCT602220.15227846867363967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTG591480.14956273230893094Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC591230.14949951684420307Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT587110.148457725985488Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAA582030.14717318774221796Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT576600.1458001478483289Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA565740.1430540680605508Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGCGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC562250.14217158017294992Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGGGAAGGTGGCTCGGGGGGGAGATCGGAAGAGCACACGTCTGAACTC555300.1404141902535155Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG554250.1401486853016585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTAGATCGGAAGAGCACACGT554210.14013857082730205No Hit
TTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACGTCTGAAC543860.13752145058756876Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCTG543210.13735709037927635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATTCGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA542180.1370966426645976Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCTG540740.1367325215877651Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAAGATCGGAAGAGCACACGTCTGAACTC532220.13457813854983977Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA508130.12848669636866347Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTCTGAACTCC501170.12672677783063996Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC497390.12577096000395477Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG481720.1218086146748127TruSeq Adapter, Index 2 (100% over 38bp)
ATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAAC474430.11996525172334839Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA472050.1193634404991392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT470850.11906000626844548No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA465970.11782604039695772Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA462880.11704469725292142Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA459200.11611416561212735Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA453280.1146172234073717Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCT450170.11383082302615717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG443950.1122580222637281Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAACT435920.11022754153666933Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA431330.10906690560426588Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC429820.10868508419730961No Hit
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC429310.1085561246492648Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCCCCGCCTCCCCTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAG427360.10806304402438752Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC409040.10343061476913017No Hit
ACATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAAC407500.10304120750640658Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTG406770.10285661834940124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGGCGATGCGGCGGCGTTATTAGATCGGAAGAGCACACGTCTGAA396650.10029765633721757Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA396070.10015099645904896Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)