FastQCFastQC Report
Mon 10 Jul 2023
SRR6880591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36867132
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG8083212.192524767047244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT5612571.5223777103138916No Hit
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT3758891.0195775467427193Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA3202130.8685595614001111Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA3046910.8264570186799449Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC2994180.8121543059004427Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGAGATCGGAAGAGCACAC2950780.8003823025886582No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA2632840.714142884778778Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA2556330.6933899821662287Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG2130370.5778507533485382Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC2107960.5717721682283287No Hit
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA2095750.5684602751307045Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAACT2009010.5449325431660916Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1760820.4776124163930083No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1497140.40609071516601836No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA1453670.394299724752118Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1440300.3906731882480037No Hit
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA1436340.38959906075688233Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT1428030.3873450204914231Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG1414810.3837591706346998TruSeq Adapter, Index 3 (100% over 49bp)
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC1397590.3790883435142175Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT1388100.3765142349559494Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA1357760.36828468241033774Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC1338540.363071366657976Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA1331630.3611970684348324Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT1316870.3571935023315619Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGAGATCGGAAGAGCACACGT1202770.32624452588283787No Hit
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA1187940.32222197267745156Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT1154270.3130891765597606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1113340.30198714670834714No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC1101980.29890581127927174Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA1100080.2983904470789862Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT1044250.28324687692007067TruSeq Adapter, Index 3 (100% over 35bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1020040.2766800520311697No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC1017110.27588530618546625Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG1003230.27212043508022266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA987230.26778052602518687Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTAGAGGAAGTAAAAGTCGTAACAGATCGGAAGAGCACACGTCTGAAC958650.2600283634756292Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG955040.25904917149508677Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC943930.25603564714499627No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTC942700.25570201663639036No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACGTCTGA919700.24946339736977644Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAA896250.24310271816098955Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAA890570.24156205044645185Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA889220.24119587061993322Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTACTTGGATAACTGTGGTAATTCAGATCGGAAGAGCACACGTCTGAA886980.24058828335222823Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACACG883820.23973115131385864No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT882050.2392510488746453Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCGAATGATTAGAGGTCTTGGGGAGATCGGAAGAGCACACGTCTGA870650.2361588636729323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT853630.23154228541563798Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA844950.22918788475328106Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCC830970.22539588921644352Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC820950.22267802117072735Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAACGAGAACTTTGAAGGCCGAAAGATCGGAAGAGCACACGTCTGAACT776860.21071885928094433Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT752320.20406252376778317Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG738680.20036275129836517Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA738070.20019729226564195Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT731590.19843962909835244Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG725310.19673621479425088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT712680.193310399083932Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCTTGGTGGTAGTAGCAAATAGATCGGAAGAGCACACGTCTGAACTC705830.1914523755197448Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC694560.18839545207910396No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA689230.18694971987514516Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAA688680.1868005355013783Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT681610.18488283818768436Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG680170.18449224637273115No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA678280.18397959461560504Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACACGTCTGAAC677850.18386295955975096Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC661820.17951491317523696No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA659620.17891817568016952No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT657600.17837026216197127No Hit
AGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCACACGTCTGAACT656780.17814784182290067Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACACG649460.1761623334302218No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAA632460.17155118005924627Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA609210.16524474971364736Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG602960.163549472739024No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTG598750.1624075341689177Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT582780.1580757624433601Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAAC572480.15528194598918083Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA566550.1536734671956582Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCTG549580.14907045115416084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT545850.14805870985570563No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG544030.1475650452006953TruSeq Adapter, Index 3 (100% over 37bp)
AAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGAA541880.14698186992142487Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG519370.1408761603696214Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCACACGTCT516650.14013837583026528Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATTGGAATGAGTCCACTTTAAATCCTTTAGATCGGAAGAGCACACGTC514520.13956062543731365No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC494550.1341438764479971No Hit
TGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCT492790.13366648645194315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGAAC487610.1322614408953753Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC486750.13202817078366713No Hit
TAAACGAGAACTTTGAAGGCCGAAAGATCGGAAGAGCACACGTCTGAACT485760.1317596389108868Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTAGCGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT484050.13129581113062985Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC483000.13101100459889314No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC473300.12837993473427767No Hit
TTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC472350.12812225263413493Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT471490.12788898252242675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG469730.12741159252637282Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC465220.1261882806614846Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC460320.12485918351337988No Hit
ACACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTG459410.12461235118587473Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG455430.12353279880843457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG455240.12348126238840601Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC452370.12270279120165897Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCCGCCTCCCCTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT452280.12267837921322439Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTAGATCGGAAGAGCACACGT443940.12041620161828699No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG435000.11799127743378572Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTTTTATCCGGTAAAGCGAATGATTAGATCGGAAGAGCACACGTCTGAA427560.1159732197231941Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT419730.11384937672938594Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC419570.11380597763883558Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCAGATCGGAAGAGCACACG414730.11249315514968727No Hit
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA411310.11156549958917336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA409060.11095519987830896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCTGGTTGATCCTGCCAGTAGCAGATCGGAAGAGCACACGTCTGAACT404920.10983224841031843Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA401880.10900766568986164Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGAGATCGGAAGAGCACACGT395530.1072852642836443No Hit
TTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACGTCTGAAC395080.10716320434147142Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGAGATCGGAAGAGCACACGT394860.10710353059196467No Hit
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA392940.10658274150536037Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACAC392800.10654476730112881No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT389310.10559812463849913Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC387560.1051234470856046Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT385110.10445889851155225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT382180.10366415266584882Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTG377020.10226453199559976Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCT376890.1022292702345276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGCGCGTCGTTCCCCTCTTCCTAGATCGGAAGAGCACACGTCTGAAC369630.10026003650080512Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph