FastQCFastQC Report
Tue 4 Jul 2023
SRR6880592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24878383
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG10382214.17318521063045Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA4464811.7946544194612648Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA4381631.7612197705936115Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3040601.2221855415603178Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG2937251.1806434525909502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2381790.9573733148171245No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2234070.8979964654455235TruSeq Adapter, Index 1 (100% over 35bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1930140.7758301654894533No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1778830.715010296288147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1609850.6470878754459243No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA1513870.6085081976589878Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT1492020.5997254725116179Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT1378240.5539909888838033Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1202310.4832749781205636Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1177610.4733466801278845No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1162630.46732538847078603Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGCGGCGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1132020.4550215341567818Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1046590.4206824856744106TruSeq Adapter, Index 1 (100% over 49bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1017940.40916646391367156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1005800.40428672554803907No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA978440.3932892262330715Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA928690.3732919458632018No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT916480.3683840706206669No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC900400.3619206280408176Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC883200.355006995430531No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT879320.3534474085393733No Hit
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC850810.34198766053243895Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA832210.33451129038410576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC828510.3330240554621255Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC824150.33127152998649473Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC797440.3205353016713345Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG775860.31186110447773074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC765080.30752802543477203No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA736460.29602406233556255Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC712260.286296742035043No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT701010.2817747439614544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT630480.25342483070543614Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG628740.25272542833672107Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA626770.25193357622961265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT613420.2465674718489542No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT603180.24245144871352775No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC595450.23934433359274193No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT591950.2379374897476255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA572080.22995063626120715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA559360.22483776377266962Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC556710.2237725820042243No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG555400.2232460204507664No Hit
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG535680.21531946027199597Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG535600.21528730384125044Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG535010.21505015016450224No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA511230.20549165112539672Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTC504580.20281864781967543No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT498980.20056769766748908No Hit
TCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC494740.19886340683797657Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA488630.19640745943978757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG485690.19522571060988972Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA482420.19391131650316662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT478750.19243614024271594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC463200.18618573401655564No Hit
TCCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT454770.18279725012674658Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT446830.17960572437525382Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA440850.17720203117702626Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC435620.17509980451703794Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCTG435190.1749269637017808Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACAC427200.17171534018107207No Hit
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA423730.17032055499748516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC417080.16764755169176387No Hit
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC412040.1656216965547962Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA411630.1654568948472254TruSeq Adapter, Index 1 (100% over 37bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG409260.1645042605863894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT399100.16042039388170845Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT395330.15890502208282586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA393150.15802875934501048Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC389220.15644907468463687No Hit
TTTCCCCCGCCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC388080.1559908455465132Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTC385180.15482517493198814No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT381700.15342637019455804Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT381260.1532495098254577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC380570.1529721606102776No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC380540.15296010194874804No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG376340.15127188933460828Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG365290.14683028233788345Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT360180.14477629032401343No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA350180.1407567364808235No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA349460.14046732860411387No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG348880.14023419448120886Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA325310.13076010607281027Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG324130.13028579871931387No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA323770.13014109478095903Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC319180.12829611956693487No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT318220.12791024239798865Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTG317650.12768112782892682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT312840.12574772243035248No Hit
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA311510.12521312176920824Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACA310300.12472675575418227No Hit
GCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC308840.12413990089307653Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAAC306010.12300236715545379Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT302680.12166385572567155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTC301350.12112925506452729No Hit
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA299590.12042181358812588Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT298190.1198590760500793Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAGCGAATGATTAGAGGTCTTGGGGAGATCGGAAGAGCACACGTCTGA291920.11733881579039925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCT290420.11673588271392074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTGACGAAAAATAACAATACAGGACAGATCGGAAGAGCACACGTCTGA288120.11581138532998708Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC283250.1138538626083536No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG278130.11179585104064038No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC273960.1101196970880302No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG273540.10995087582661622Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACACGTCT271160.10899422201193702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT270390.1086847163660114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT268640.10798129444345317Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC267010.10732610716701324No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC259440.10428330490771848No Hit
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC258530.10391752550798819Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGTGCGGGTCATAAGCTTGCGTTGATTAGATCGGAAGAGCACACGTCTG257880.10365625450818086Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGAAC254720.10238607549373285Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATTGGAATGAGTCCACTTTAAATCCTTTAGATCGGAAGAGCACACGTC250330.10062149135657249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)