FastQCFastQC Report
Tue 4 Jul 2023
SRR6880593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30127976
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG9269303.0766421215948925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA7266092.4117418309148944Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7000682.323647629034224Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA6594012.1886667727032174Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG3870221.284593429044155TruSeq Adapter, Index 2 (100% over 35bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT3020461.0025432840227966Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG2781630.9232714471094906Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2567030.8520419692315209No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC2158250.7163607671487789No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT2071340.6875138243604549Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGCGGCGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2026220.6725377104655156Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT2025580.6723252833180695Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1861220.6177713365146069Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1856820.616310899875916Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1856660.6162577930890545TruSeq Adapter, Index 2 (100% over 49bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1849640.6139277328155067Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA1768750.5870789328828462Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1628980.5406868353851583No Hit
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1549670.514362464972755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT1544630.512689601186618Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA1472400.4887152060928354Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1407740.46725342585243695No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1404990.46634065295325516No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1319540.4379783095950422TruSeq Adapter, Index 2 (100% over 37bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1300350.4316088143458426Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1291010.4285087056628032Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1256280.4169812137396817No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1234540.4097653290748771Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC1192710.39588122348477706Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC1190830.39525721873915465Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1083150.35951635118137376No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA1020710.33879142760867836Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1005910.33387904982399086Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT990970.32892020360079943Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA976650.3241671461766964Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA973600.32315479805214925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG935720.3105817662626922No Hit
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG928660.30823842929242906Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT829400.275292306393234Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA829050.2751761352969745Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG821470.27266020126941154No Hit
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT819700.2720727074397563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC816230.27092095399969784No Hit
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA815510.27068197345882117Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG809040.2685344677651097Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC783070.2599145724226546Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC745370.2474012857684167No Hit
TCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA724190.24037127485762735Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA722260.23973067424111066Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT706640.23454612417375797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC683160.22675270320183472No Hit
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG682000.22636767899708896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG678620.22524579812464005Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGATCGGAAGAGCACACGT649320.2155206177806302No Hit
TCCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT631650.20965563700661471Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC628350.20856030952759655Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG605650.20102578414162306Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA593500.19699298751432887Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG581230.19292036079688857Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT578380.19197439615591835Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA573120.19022851053784695No Hit
TTTCCCCCGCCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC568080.1885556467517101Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT564630.1874105316600093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC558960.18552855990060535No Hit
TCGGGGTCGGGGCACGCCGGCCAGATCGGAAGAGCACACGTCTGAACTCC556870.18483485249722717Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC551240.1829661574345386No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG548750.18213968306400669Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT542190.1799623048026857No Hit
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA534490.17740654068497666Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG529680.1758100179049532Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTC519420.17240454519746035No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT512360.1700612082271972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG496080.1646575926640409Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC488550.16215825450737215No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC488200.16204208341111265No Hit
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT475740.15790639238427434No Hit
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA473560.15718281241328658Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCTG471050.156349699694397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTAGCGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT468350.1554535226661094Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT460320.15278822580049853No Hit
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC447090.1483969583618893Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC446740.1482807872656298No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACAC440490.14620630340385296No Hit
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA437230.14512425262155015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT436840.14499480482857527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT427310.14183163183613795No Hit
GCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC425840.14134371323184805Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT424630.14094209315620804Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA422660.140288215842976Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG419440.13921944175738854No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTC405320.13453276781686233No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC402540.13361003739514396No Hit
AAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTC395200.13117376354787325No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG392500.13027758651958565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC390290.12954404902606134Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC389980.12944115462651723No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA388400.12891672510626004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG386830.1283956147601817No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC381690.12668955923225644No Hit
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC381270.12655015391674504No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA368890.12244101628333746No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT366650.12169752126727662No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC365080.1211764109211983Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA363320.12059223626572192Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT349080.11586573223504956Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT328160.10892201985291013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA326300.10830465345564534Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT323650.10742507229825196No Hit
TTGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT319250.10596463565956107Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT318800.10581527282151314No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA307860.10218409626985894No Hit
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA307580.10209115939285136Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACA305890.1015302189566269No Hit
ATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACTC302740.10048467909029137Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA301930.10021582598180509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)