FastQCFastQC Report
Fri 7 Jul 2023
SRR6880594.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880594.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22889605
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6477332.8298129216297094Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG4840902.114890143364204Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT3892991.7007676628757902TruSeq Adapter, Index 3 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2954631.290817381951327TruSeq Adapter, Index 3 (100% over 49bp)
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2527711.1043047706589957Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2359041.0306162994075259Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2327651.0169026507884256Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGCGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2006990.8768128589374958Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT1975330.8629812528438128Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG1873520.8185025473353517TruSeq Adapter, Index 3 (100% over 37bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG1838060.8030107990067981Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA1723130.7528002339926793Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT1676490.7324241724573229Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1673890.7312882856650432No Hit
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT1627320.7109428056971713Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1507650.6586614316848194No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1320560.5769256393895832No Hit
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC1291020.5640202179111435Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA1267220.5536224849664291Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT1222590.5341245512974121Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1187290.5187027036945373Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC1121080.4897769096495985No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT1079550.4716333025406074Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC979180.427783703563255Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC974380.4256866817928925Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT941530.4113351890519735No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA891350.38941257396097484Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG861780.3764940460964704No Hit
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC828190.36181926249928736Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC813200.35527043826225924Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT770160.3364671430546748Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT722440.3156192516209869Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG704330.3077073632332231No Hit
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA698900.30533510735550046Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG674340.29460534596381194No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA656310.28672840793888754Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC631520.27589816425403585No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACAC621320.2714419929920153No Hit
TCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC606150.26481453043859865Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC599890.26207966454641746Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG596970.26080397630278024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA594040.2595239192637881Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA584620.25540851403945153Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC580630.2536653646928377Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG535650.23401452318639837Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG526750.23012629532051776No Hit
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT510480.22301826527805962Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT501490.2190907182539847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG497790.21747426397266356Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC495700.21656118574348487No Hit
AAGTGCGGGTCATAAGCTTGCGTTGATTAGATCGGAAGAGCACACGTCTG488940.21360788008355758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC483850.21138416324790227No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA474790.2074260346563429Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAACGATCTCAACCTATTCTCAAACTTTAGATCGGAAGAGCACACGTCT472930.20661343872032742Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC467520.2042499204333146No Hit
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG449750.19648657108761813Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC444910.1943720741358359No Hit
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA442820.1934589959066572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACA440250.19233621550044225No Hit
GCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC439530.19202166223488784Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACAC432200.18881933523973No Hit
AAATTGGAATGAGTCCACTTTAAATCCTTTAGATCGGAAGAGCACACGTC432150.1887974912629554No Hit
TCCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT428330.1871286114373752Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC423220.18489615701101003No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT418740.18293893669200495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA416950.1821569223234739Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG406990.17780560214997157Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA406770.1777094886521633Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC402080.17566052363070486Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT389170.17002040882750052Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG384400.1679364934432027Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC383860.16770057849403694No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGAGATCGGAAGAGCACAC373460.16315703132491802No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT370960.16206483248618753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTC355860.15546795150025525No Hit
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT350650.15319180912034086No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC349350.152623865724201No Hit
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA345710.1510336242150094Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA345540.15095935469397573Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA345400.1508981915590068TruSeq Adapter, Index 3 (100% over 36bp)
TCGGGGTCGGGGCACGCCGGCCAGATCGGAAGAGCACACGTCTGAACTCC343240.14995453176234366Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC341560.14922057414271675No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG336690.14709297080486974Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTAGCGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT336310.1469269565813827Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT334030.14593087124046045No Hit
TTTCCCCCGCCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC333060.14550709809103304Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAGCGAATGATTAGAGGTCTTGGGGAGATCGGAAGAGCACACGTCTGA329100.14377705513048392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG325630.14226108314232597TruSeq Adapter, Index 3 (100% over 38bp)
AAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTC324110.1415970262483778No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC323750.14143974961560063No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT317090.13853013190922256No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG309950.13541081202580824Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA304180.13289001710601822No Hit
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCTG300190.13114686775940432Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG294380.12860859765819463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG293620.12827656921122055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC289660.12654652625067142No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTC284460.12427475266611196No Hit
TAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCTG278960.12187191522090486Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA273710.1195782976595708Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT273010.11927248198472626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC272550.11907151739839984Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC266970.11663372959035335Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTAGCAGAGCAGCTCCCTCGCTGCGATCTAGATCGGAAGAGCACACGT257000.11227804062149609No Hit
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC252700.11039945861887962Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT247140.10797040840154297Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG246970.10789613888050929No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT244580.10685199679068293No Hit
TCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGCAGATCGGAAGAGCACA243340.10631026616667259No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT242000.10572484758911305Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT233510.10201574033278425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC233470.10199826515136455No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG230650.10076626486127656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)