FastQCFastQC Report
Fri 7 Jul 2023
SRR6880595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25312746
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC9950123.9308734026723133Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG8259463.262964832025731Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG7020742.773598723741786TruSeq Adapter, Index 4 (100% over 35bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC6904692.7277522557212874TruSeq Adapter, Index 4 (100% over 37bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA3900731.5410141594278235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA3562441.407370026152042Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGCGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT3084181.2184296401504602Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT2836751.1206804666708228Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2452000.9686819438712814TruSeq Adapter, Index 4 (100% over 49bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC2195150.8672113250770975No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2090330.8258013571502674No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT2037680.8050015592934879Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG1919820.7584400364938676Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC1593780.6296353623585524TruSeq Adapter, Index 4 (100% over 38bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1559780.6162033941319524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1412300.5579402566596291No Hit
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1374870.5431532398736983Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT1359010.5368876217538785Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA1357840.5364254040237278Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1300840.5139071043497216No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1235690.4881690828802217Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC1148220.45361336932784774Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1141150.4508203100524929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1080460.4268442467680117Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1063610.4201875213380642Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC995080.3931142042036846Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC967510.38222245820346795Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG900410.3557140738503835No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC891730.3522849713737103No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA887020.3504242487164372Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA843400.3331918235974872Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT835630.33012222379982004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT830300.328016565251356Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG803290.3173460516689892Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA791570.31271597320970235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA770150.30425383322694427TruSeq Adapter, Index 4 (100% over 36bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC758680.2997225192399118Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA696410.2751222644907826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC678630.2680981352240488Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG646220.2552943090409867Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT620840.24526773981771874Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA617190.2438257785228043Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT616600.24359269436828387Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG607350.23993840889487061No Hit
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC606630.23965396721477789No Hit
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG595180.2351305543855258Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT584090.23074936239632No Hit
TCCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT571000.22557805462907898Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC569180.2248590492710668TruSeq Adapter, Index 4 (100% over 37bp)
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA561720.22191191741899513Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT550600.21751887369311887No Hit
TTTCCCCCGCCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC540990.21372236737965924Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA539880.21328385312284967Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA536850.21208682771912618Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT522290.20633478485502918Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG520010.2054340528680689Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG515960.20383406841754742No Hit
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT513770.20296889164059875Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG496030.1959605646894256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT494930.19552600101150625No Hit
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG479200.18931174041725854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC477040.18845841537698044Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC473730.187150773764332No Hit
GCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG469570.18550733294601857TruSeq Adapter, Index 4 (100% over 35bp)
TCGGGGTCGGGGCACGCCGGCCAGATCGGAAGAGCACACGTCTGAACTCC469040.18529795226483922Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG453250.17905998819725052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT450970.17815925621029027No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC448540.17719926553997736No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC441340.1743548487390503No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA439840.1737622619055238No Hit
TCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC439510.17363189280214797Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC437590.17287338165523408Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT431490.17046352853222643Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCTG429780.16978797954200622Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTC418400.16529222076498534No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT415990.16434013125245284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAGCGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT412070.16279150432750364Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC395350.1561861364231285No Hit
TTTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT390830.1544004747647687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA389860.1540172686124216Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG388630.15353134740892987No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG388420.15344838525223617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG381760.15081729971137858Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC379060.14975064341103095No Hit
ACGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC377130.14898818168522687Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC375120.1481941153283014No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG373780.14766473775701777Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT365040.14421193180700348Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA355360.14038777144131262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC353050.13947518771768183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA349860.13821495305171552No Hit
TTGGCGGGGGCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG343510.13570633545645344TruSeq Adapter, Index 4 (100% over 35bp)
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA341330.13484510925839496Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT337100.1331740143878503No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG336600.13297648544334145No Hit
TCGTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT334640.13220217198086687Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA329260.13007676053795192TruSeq Adapter, Index 4 (100% over 36bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA326600.12902590655316495Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTC319830.12635136464451546No Hit
TTAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCT309780.12238103285988806Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT309550.122290169545414No Hit
AAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTC308710.12195832091863917No Hit
TAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCTG293250.11585072595442629Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA293050.11577171437662276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT283670.11206607137763719No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA278070.10985374719913833No Hit
AAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGAA273710.10813129480302136Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG273080.10788240833294026TruSeq Adapter, Index 4 (100% over 35bp)
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC272530.10766512649398054No Hit
CGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT270870.10700933039821123Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAGACTCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT270510.10686710955816489Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGAAC267260.10558317141885752Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC267130.10553181389328521Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTC261540.10332344029367656No Hit
TTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAAC257130.1015812350031087Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACACGTCT256030.1011466713251893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA255920.10110321495739735No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)