FastQCFastQC Report
Tue 4 Jul 2023
SRR6880596.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880596.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23353932
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG17231587.378449162222448Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA6062482.595914041369993Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA5738942.457376342450599Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA5420072.3208383067999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC4397301.8828949232189252No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG4072621.7438690838013915No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3639881.5585726634812502No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT2719601.1645148234567095No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT2655711.1371575458899168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG2193170.9391009616710367Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG2118360.9070678119641694No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1895400.811597807170116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1623350.6951077874166971Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG1503590.6438273435068664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1331000.5699254412490368No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1269420.5435572904811061No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC1250020.5352503381443433No Hit
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1185240.5075119684342663Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1171680.5017056656669207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1153220.49380121514441333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA1115240.47753842907481275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1069820.4580898839647216Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1030320.44117624389760146Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT988790.4233933711890571Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG897930.38448771710048657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC895090.3832716477893316No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT885960.37936224187002No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG870770.3728579838290186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT777900.3330916609674123No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA770910.33009858896566113Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT748860.3206569240674333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA747460.32005745328024415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG745080.31903835294202276No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT743590.31840034474708584Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT733630.314135538289655No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC706150.3023687831239724No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT687330.2943101829704737Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT683510.29267448410828634No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC680440.2913599303106646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC646520.2768356095239123Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG628770.26923517632919375No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC598180.25613673962911254No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA583150.24970099253521846Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC567470.24298691971870093Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT555240.23775011419918496No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA552210.23645268813834003Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA549050.23509959693297044Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA530640.22721655608143415No Hit
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC525230.22490002968236783Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT520400.22283185546656553No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT461800.19773972108850876Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT457740.1960012558056605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA456100.1952990185978104Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG452300.19367188360401152Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG439860.18834515746641722TruSeq Adapter, Index 5 (100% over 49bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA422110.18074472427169866Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA395970.1695517482880399Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC386970.165698007513253No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG385640.1651285102654234No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT380070.16274347291924976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC369140.1580633188449808Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC366860.15708703784870146No Hit
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG362790.15534429063165894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA362730.1553185990264937No Hit
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA350350.15001756449406464Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC339060.14518326078880422No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA322840.13823796352579942Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA318450.13635819441454228No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCAGATCGGAAGAGCACACGTC312840.13395602933159179No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC311200.13325379212374172No Hit
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG307930.13185359964223584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT305930.1309972128033943Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG296340.1268908379111492No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG283760.12150416469483598Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGT270140.11567217032232516No Hit
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT269780.1155180206913337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG268280.11487573056220256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG267590.11458027710280222Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAA267190.1144089997350339Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA263220.11270907185993348Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT262410.11236223519020265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTGA262220.11228087844051271Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG259060.1109277872351431No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT255750.10951046701686037No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT254290.10888530462450606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA254090.1087996659406219Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAACTC247280.10588366875436651Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTG246180.10541265599300366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA239230.10243671172802936No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC239090.10237676464931043Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCGGCGCCTCGCCTCGGCCGGCGAGATCGGAAGAGCACACGTCTGAAC237190.101563197152411Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC236380.10121636048268016No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)