FastQCFastQC Report
Tue 4 Jul 2023
SRR6880597.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880597.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23128798
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG16853377.286747024207657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA6644252.87271738029793Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA6006332.5969053817669208Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG5461322.3612640829843388No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG4678562.022828856043448No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA3793861.640318705710517Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT2912161.259105639644568No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2856611.2350879626342883TruSeq Adapter, Index 6 (100% over 49bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC2769981.1976324926180772No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2370751.0250208419823632Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG2089250.9033111015972382Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1983190.8574548491452085No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1865310.8064880846812705Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1822580.7880132811052265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1564140.6762737951189681Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1545860.6683702283188258Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1406540.6081336349601912Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1282530.5545164949773871Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG1229420.531553779837586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1225630.5299151300469657No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1023250.4424138253963738Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1001840.43315696734434705Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG992690.42920086033005256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT982910.4249723656196919Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT931470.4027316940551775No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC860150.3718956774147969No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG856690.37039970689354457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC831480.3594998754366742No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG823520.3560582785149492No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA807480.34912320130081986No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC806200.34856977868024097No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT800610.34615287832943153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT788540.3409342759619415Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT769560.33272805616617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA726920.3142921651181354Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA692930.29959620037323165Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG675360.2919996101829416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT674330.2915542779179445No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA674140.2914721292477024Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC669340.2893967944205315TruSeq Adapter, Index 6 (100% over 35bp)
TCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT668700.28912008311024207Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT642760.2779046278150728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT635330.27469218244718124No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC621850.2688639504742097Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG614270.265586650892969Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT592250.25606605237332264No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT576680.24933418502768712Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT562930.24338921547068723No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC554270.23964496555333314No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA543280.23489331352195647No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA538380.23277474255255287Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT529450.2289137550511704Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC521430.22544621644410576No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG519110.22444313794430648No Hit
ACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAG507940.21961366085691092Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT490240.21196086368171835Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC464630.2008880876559171Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA404160.17474319244778738Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC393320.17005639462975985Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA393030.16993100981728493No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC369160.15961054266633312No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT353790.15296514760516305No Hit
TCGCGGCGCCTCGCCTCGGCCGGCGAGATCGGAAGAGCACACGTCTGAAC353580.15287435170647432Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA351840.15212204283162487Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG334480.14461624854002356No Hit
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG328400.14198749109227382Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC327790.14172375062465417No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC325620.14078552633820401No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC322890.13960517965525057Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG322540.13945385315743603No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT319370.1380832674486586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA314410.13593875479391535Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC311360.1346200524558172No Hit
AAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC299890.1296608669417235Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA296260.1280913949786755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACT295330.12768929885591115Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA288210.12461088552894102Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTG283040.12237557697550906Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG280400.12123414282056509No Hit
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG275780.11923663304941312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG272540.11783578204107277No Hit
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA261310.11298036326833759Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT260960.11282903677052306No Hit
ATCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA259650.11226264330727433Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA259290.11210699319523652No Hit
TTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC255000.11025216269345256No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG254790.11016136679476382Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG247000.10679327131483443Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCATCTGTGGGATTATGACTGAACGAGATCGGAAGAGCACACGTCTGAA246070.10639117519207007Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC243920.10546159813406646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT243830.105422685606057Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG241990.10462714058897483Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT240800.10411263049640539Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA236070.10206756096879743Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTC234830.10153143280511162No Hit
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCTG233810.10109042415433782Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA233500.10095639211341635Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA232440.10049808900574947Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)