FastQCFastQC Report
Tue 4 Jul 2023
SRR6880598.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880598.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24234673
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG16548276.828344661386601Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA5348662.207027922349107Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA5265882.1728702508179087Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG3880931.6013956532444238No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA3878611.6004383471565717Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC3830891.580747551246101Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC3461091.428156261897984No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA3452631.4246653957327997TruSeq Adapter, Index 7 (100% over 35bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3286871.3562675262835193No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2931841.20977081060677TruSeq Adapter, Index 7 (100% over 49bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT2898371.1959600197617686No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT2087670.8614393105283492Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1810130.7469174434497218Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC1773830.7319389042303149No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1699940.7014495305961009Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1648460.6802072386122149No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG1599050.6598190947325759Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1584680.653889573834976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC1380550.5696590170620417No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1336180.5513505381318742No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1334570.5506862007174597Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1088400.4491085974215538No Hit
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1076260.4440992457377081Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1073220.44284484465707463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1054260.43502134318049185TruSeq Adapter, Index 7 (100% over 37bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1043790.43070108682712577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1040420.42931051720813396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA1016270.419345455991917Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT954960.39404699209269295Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT919110.37925413724377466Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG841700.34731229920040596Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC793460.3274069346840372No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG773330.31910065384418435No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT754310.3112523944515364Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA752220.3103899937086009Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT746100.3078646862699571No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG710400.2931337262112016No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT707140.29178854610499594No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT616700.25447011395614866Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT609190.25137124812866257No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC608580.25111954264866704Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA603380.24897385658968868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG581880.24010226999968187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA573750.236747572372856No Hit
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC550980.2273519432261372Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG544930.2248555200229027No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC543420.22423244580193016No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA536890.2215379592701746Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT527150.21751892422893432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA522270.21550528038897Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT485640.2003905726312049No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT485270.20023789881546986No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA451220.18618778144850562No Hit
AAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC447880.1848095907875464Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC445870.1839802005993644Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC434550.1793092071017422Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC426320.17591324628147448No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACA392250.16185487627582185No Hit
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA390490.16112864407124453Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA388290.1602208538155229Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT382350.1577698201250745Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG377530.1557809342011753Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA375190.15481537547463503No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT372570.15373427980645746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT366780.1513451409061719No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA358490.14792442216983906No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA350050.14444180864334336Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT337010.13906108821852062No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG334520.1380336347018175Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG333790.13773241338969172Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC326600.13476558978121966Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT314450.12975211177802978Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA308920.12747025718069313Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA301240.12430124392435582Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA301170.1242723596889465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCCCGTCCTTTCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT299040.12339345366863419Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT298660.12323665353355501No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC295610.1219781261335773No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGT293090.12093829365884161No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG286630.11827269136249538Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC285510.1178105435959462No Hit
TTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC285150.11766199609955538No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC280380.11569374177237712No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC279840.11547092052779091No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG276460.11407622458945496No Hit
TCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAACTC274290.11318081329176588Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC271540.11204607547211387No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC267260.11028001079280086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT263470.10871613576135315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA261700.10798577723743166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT261360.10784548237972925No Hit
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG259510.10708211330105424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG258820.10679739726630517No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC250050.10317861520145123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)