FastQCFastQC Report
Fri 7 Jul 2023
SRR6880599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26332345
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG13853415.2609860610591275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC12675304.813585725084492TruSeq Adapter, Index 5 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG6857442.6041888787344996TruSeq Adapter, Index 8 (100% over 49bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA6320872.400420471477189Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA4783931.8167504641155203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT4759921.8076324003805968TruSeq Adapter, Index 8 (100% over 37bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA4742201.8009030338923477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC4399011.6705728259294794No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG3455531.312275834149978No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT3193001.2125771555856495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2922991.1100378640793291Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT2489350.9453582656614896No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2403270.9126684311632709No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1872260.7110114955580296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1752080.6653718079419056Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC1478730.5615641143999898No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1349990.5126736718662922Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1317860.5004719480927354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1262660.4795091360074464No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT1232470.4680441487455827No Hit
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1172470.44525848343548596Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1152380.4376290831674885No Hit
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1110140.42158797478918036Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG1104840.41957524102012184Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG1009580.38339919972945824Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG989270.37568625202199046TruSeq Adapter, Index 8 (100% over 38bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC945550.35908309723269993No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG913540.3469269447897633Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG872660.33140231149181737Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT840450.3191702068311804Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA837360.3179967450677105Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT830670.31545614338563466Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC780640.296456696127899No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG764990.2905134350928487Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC743930.2825156665690048TruSeq Adapter, Index 5 (100% over 35bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT712670.27064433494244433No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG698510.26526691792926155No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA692730.2630718988377222Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT683410.2595325254928872Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT673500.2557690931058362Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG671670.2550741303138782No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA667460.2534753361312864Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC635470.24132677891011986Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT633640.2406318161181619Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT600880.22819084285884908TruSeq Adapter, Index 8 (100% over 36bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG597560.2269300360450237No Hit
ACGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC569460.2162587494581284TruSeq Adapter, Index 5 (100% over 35bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC551700.20951419252633977No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA540550.2052798563895468No Hit
AAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC535780.20346839599739408Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT523570.1988315131067894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT522480.19841757352032263No Hit
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC514610.1954288537538149Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA481120.18271065489989594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC445160.16905444615737794Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA438150.1663923209269816Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT436670.16583027451599924No Hit
ACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT428780.16283395952772153TruSeq Adapter, Index 8 (100% over 37bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC414970.15758945889551423No Hit
TTCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTC406760.154471620358916No Hit
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC400590.15212849444286106Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG395050.15002461801256212Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA394720.1498992968533566Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGT365270.1387153327969841No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT364820.13854444030715837Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT349600.13276447654016382No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG345010.1310213731439414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC334830.12715540526299499No Hit
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA321830.12221851111247403Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG320180.12159190531644637TruSeq Adapter, Index 8 (100% over 38bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG320030.12153494115317114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC319110.12118556095174965No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA316990.12038046744412623No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG311800.11840950739480285Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA309940.11770315177018985Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC305180.11589548898892219No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC304260.1155461087875007No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC303950.11542838285006518Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT301090.11434226613695059No Hit
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA293940.11162697435416406Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA289670.11000539450626216No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT289230.10983829962732145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAA289090.10978513307493122Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA288680.10962943102864556Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA288500.10956107403271528Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG279190.10602549829876527Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCCCCGCCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC277000.10519382151494673Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA270920.10288487409685693Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG268080.10180635260551235Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCCGCCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC266260.10111518742443942TruSeq Adapter, Index 5 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)