FastQCFastQC Report
Fri 7 Jul 2023
SRR6880600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30100134
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG28483879.463037606410655No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC27916859.274659707494989No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG10384493.4499813190200417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT5087991.6903546010791846No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT4251151.4123359052155715Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT4130351.3722031935140222No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCT3418281.1356361403573818Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2585320.8589064753000768No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC2571640.854361645034537No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA2336320.7761825910808238No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG2328910.7737208080203231No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTG2281370.7579268583987036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2075550.6895484252661467Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC2048560.680581687775875No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1815780.603246483886085No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1747920.580701733753079Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1726090.5734492743454233TruSeq Adapter, Index 5 (100% over 49bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG1710400.5682366729663064No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT1687370.560585544237112No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1623020.5392069018696063Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1570710.5218282416948709Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC1510840.5019379647944424No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC1495000.49667552975013335No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1355600.4503634435647363No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1340150.4452305760499272No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA1297910.4311974159317696No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA1216380.40411115777757006No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1189030.3950248194908368No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG1176310.39079892468252797Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1094160.3635066873788668Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC1084720.3603704887160968No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA1073280.35656984118409574Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGAGATCGGAAGAGCACACGTCT982740.32649024087401074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTC972950.3232377636591252No Hit
ACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG951430.31608829382620024TruSeq Adapter, Index 5 (100% over 37bp)
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA932520.3098059297676216Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC888890.2953109776853485No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC884980.294011980146002No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACG853740.2836332888086146No Hit
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC829320.2755203681153047Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC789180.2621848793098396Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC773030.2568194546907997Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG725110.2408992597840262No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC711890.23650725275840964No Hit
TCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT664000.2205970245846746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA652240.21669006523359663Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCT643490.21378310143071125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG634100.21066351398967195No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT624580.20750073737213262No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA594830.1976170604423223No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG582310.19345761052093655TruSeq Adapter, Index 5 (100% over 37bp)
AAATGCAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA566130.18808221916885817Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCA563550.18722508012755026No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG559030.18572342568308833No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGAGATCGGAAGAGCACACGTCT557890.1853446898276267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA532630.17695270060923982Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC531450.17656067577639353No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCAGATCGGAAGAGCACACGTCTG519650.17264042744793096Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTG510440.16958064040512247Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC505650.1679892853633143Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA502640.16698928981512176No Hit
ATGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG502540.16695606737166022No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT498320.1655540802575829Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACCCCGCGGGGGTCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCT497180.16517534440212128Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA490650.16300591884408222No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTCT489330.1625673825903898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC487780.1620524347167358Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC478520.15897603645219652No Hit
TAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC468280.1555740582417341TruSeq Adapter, Index 5 (100% over 35bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT466310.15491957610554158No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGAGATCGGAAGAGCACACGTCTGA463410.1539561252451567Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC435540.14469703025242345TruSeq Adapter, Index 5 (100% over 35bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA425330.14130501877499946No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT417110.1385741339224603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA411220.13661733200257514Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGTAGATCGGAAGAGCACACGT409340.13599275006549805No Hit
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG388130.12894626980730384Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA387980.12889643614211155Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGGCGAGTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGT379340.12602601702703384No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG375970.12490642068237968No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAGATCGGAAGAGCACACGTC366100.12162736551272496No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT363360.12071707056187857No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC361380.12005926618133993No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT353460.11742804865918538Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGC353220.11734831479487766No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG352850.11722539175406993No Hit
ATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAACT351140.11665728797087746Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC345750.11486659826830006No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA343790.11421543837645373No Hit
AAACCCCGCGGGGGTTCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTC340860.1132420207830304No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG332510.11046794675399119Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCAGATCGGAAGAGCACACGTCTGA330570.10982343135083718Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCCGCGGGGGTTCCGGAGCGGGAGGAAAGATCGGAAGAGCACACGTC329990.1096307411787602No Hit
AACCCCGCGGGGGTCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCTG321950.1069596567244518Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC321420.10678357777410559No Hit
TCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT318020.10565401469641297Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG313950.10430186124752801No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC313170.10404272618852793No Hit
AACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA313170.10404272618852793TruSeq Adapter, Index 5 (100% over 36bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC311410.10345801118360469No Hit
CGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTG301880.10029191232171925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)