FastQCFastQC Report
Tue 4 Jul 2023
SRR6880601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31460514
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG331932110.550752603724147No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC27547818.756312754457857No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG8357732.6565777024494897Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT5695211.8102723941509666No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT4566851.4516132826056178No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG3417381.08624417261587No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2935740.9331506789749207Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2851220.906285256496445No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC2750310.8742101289254206No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG2698790.857834045559459No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT2483180.7893005181034233No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCT2406910.765057430403076Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2317790.7367298576240681TruSeq Adapter, Index 6 (100% over 49bp)
ACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA2236300.7108275471913778TruSeq Adapter, Index 6 (100% over 37bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2197570.6985168773784179Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG2073550.6590960338410237No Hit
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA2068560.6575099186237072Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2022520.642875701267945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2021870.6426690930733046No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA1812120.5759982179566424No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC1809890.5752893929196453No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1708330.5430076571539804No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1572690.4998932948139373Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTG1531890.4869246573657379Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1521770.48370792670456686Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1456730.46303439289008436TruSeq Adapter, Index 6 (100% over 37bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC1376850.43764383506258037No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1349570.428972648062902No Hit
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1340240.4260070258229093Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC1332250.42346733432263695No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1252480.3981117409588413TruSeq Adapter, Index 6 (100% over 35bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1244520.39558158522139847No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1209880.3845709577408684No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACG1201970.3820566949414749No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT1139230.3621142362772585Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1117230.3551213435355824Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1060570.33711146613815657No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1056860.33593221013490115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1024110.3255223357126333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG1001900.31846269263115023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC971070.3086631070299742No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGAGATCGGAAGAGCACACGTCT961600.30565298456344353Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC956250.3039524401921723Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG952990.3029162206313603Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA935980.2975094431070007Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC903510.2871885691378087TruSeq Adapter, Index 6 (100% over 35bp)
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA889490.2827321893087952Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA854690.2716707044265075No Hit
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC825750.2624718718835935Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT808280.25691887932918067No Hit
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTC796190.2530759669088687No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT785930.24981473602115972Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGCAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA720320.22896002271291563Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAGATCGGAAGAGCACACGTC709260.22544450481641842No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA676640.21507595203307867No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC666460.21184014984624855Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA605130.19234587203502143No Hit
TAACCCCGCGGGGGTCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCT603180.19172604745110014Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC576870.18336318344957747No Hit
TAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA561240.17839505101537756Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT558400.17749233213417936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG550430.17495899780912672No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA549870.17478099690297497Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT541430.1720982689602592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA539560.17150387307721673No Hit
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA539440.17146573002589852Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG536840.17063929724733679Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT529030.16815682032404175Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC503940.1601817440109211TruSeq Adapter, Index 6 (100% over 36bp)
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCT503180.15994017135257232Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG499870.15888805885371102Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC499680.15882766568912382No Hit
ACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAACTC493690.15692369171082202Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG485940.15446028631318612No Hit
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACAC478280.15202548820403888No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACA463480.14732117854145677No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA456420.1450770956889007No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG455700.1448482373809913No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC430780.13692719705723816No Hit
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA429780.13660933829625288Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC425140.1351344736452812TruSeq Adapter, Index 6 (100% over 36bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC419990.133497501026207No Hit
TCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA418590.13305249876082761Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGAGATCGGAAGAGCACACGTCTG415560.13208938671504222Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGGGCGGCGGGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA409560.13018223414913055Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG401210.12752811349490348No Hit
ACGGGGGCGGGCGGGCGTCCCGCGCGTGAGATCGGAAGAGCACACGTCTG395870.12583074771124209Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCAGATCGGAAGAGCACACGTCTGA391930.1245783841929601Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG389390.12377102294005748No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC387300.12310669812959826No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT386820.12295412592432534No Hit
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG380180.12084354375138308Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC379920.12076090047352692Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA377440.11997261074628342TruSeq Adapter, Index 6 (100% over 38bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG376270.11960071599593064Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGC366490.11649205731349463No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT357250.11355504236199064Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC349780.11118063741743063TruSeq Adapter, Index 6 (100% over 35bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAGATCGGAAGAGCACACGT345840.10992827389914864No Hit
TCGGCGGCGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG345730.10989330943544023Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC338720.10766511952093344Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC327840.10420681620141362No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC325770.10354884856617408No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT324760.10322781121757897No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT323310.1027669160141503No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT320420.10184830419490286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)