FastQCFastQC Report
Tue 4 Jul 2023
SRR6880602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28376177
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC617935421.776555735467817No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG14079214.9616303140482945Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT13722834.836039047825223No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG8007182.8217966077671424No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG7126772.5115328255811207No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3749891.3214923208295466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC3302471.1638178039275693No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2902741.0229496383533272TruSeq Adapter, Index 7 (100% over 49bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2292820.8080087744025561Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT2039830.7188530012340986Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC1918730.6761763573718899No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1580660.5570376869301316No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT1491600.5256522046644974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1344140.47368607829025033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1214670.4280597770446667TruSeq Adapter, Index 7 (100% over 37bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1139460.4015551495890373Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC1106240.3898481462108162No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1084680.38225022348852705No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1068170.3764319626283696No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1066140.37571657380062157No Hit
TCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT958730.33786439942209273Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT945910.3333465251503048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA938030.3305695478288002No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCT931090.3281238342994548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA902960.3182105891149467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT886150.3122866057679299No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA852950.30058665055549944TruSeq Adapter, Index 7 (100% over 35bp)
ACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA771000.27170679122843083TruSeq Adapter, Index 7 (100% over 37bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC769860.2713050457783654Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC744230.2622728213176849Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA702220.24746814907448594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA680910.2399583284245795Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA680370.23976802794823276TruSeq Adapter, Index 7 (100% over 35bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGTAGATCGGAAGAGCACACGT673660.2374033683254795No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG669740.2360219278305178No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG629660.22189740358611382No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA597890.2107013922277127No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTG592810.20891115811689503Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA565900.19942785104561478Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG562280.19815213303751245TruSeq Adapter, Index 7 (100% over 36bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG547910.1930880259169514No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG546730.19267218413530476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT532650.18771027541870774No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC525780.1852892304696295Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA523480.18447869140370812No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC515030.18150084135717084No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG501350.17667989595638622No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC463340.16328485687131145No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT462550.16300645432258193No Hit
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG446220.15725162695454006No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC421630.14858590711497183No Hit
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG410990.1448362829143616No Hit
ATCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT398000.14025849923335337No Hit
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT390880.13774935221189238TruSeq Adapter, Index 7 (100% over 38bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCCAGATCGGAAGAGCACACGTC383420.13512038637199084No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG381790.13454596086005527No Hit
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG371710.13099368530158237No Hit
TTTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG370810.13067651784100445No Hit
TCGGCGGCGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC370410.13053555452519203Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCAGATCGGAAGAGCACACGTCTGA370300.1304967896133436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTCTG364860.12857968851829477Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG360630.12708900145357846No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA358060.1261833121494837No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT353570.12460099892948934No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG350530.12352967772931499No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGAGATCGGAAGAGCACACGTCT349950.12332528092138698Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG343640.12110158461444613No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG341080.12019941939324667No Hit
GTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT339980.11981177027476252No Hit
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTC339090.11949812689707989No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAAATCGGAAGAGCACACGTC335460.11821888480608224No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAAGCACACGTCT326000.1148851023871186No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC325830.11482519297789832Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTC322740.11373625136324743No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC313040.11031789095479634No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT312600.11016283130740269No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA310820.10953554455203744No Hit
TCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC309830.10918666034540171Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGGGGCGGCGGGTCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT296680.10455249133806854Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC290370.10232879503112771No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)