FastQCFastQC Report
Tue 4 Jul 2023
SRR6880603.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880603.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13958802
Total Bases697.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2966662.1252969989831505No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2672411.9144981066426763Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2379851.704909919920062Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2111821.5128948745028405Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT1783771.2778818698051595No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT1486731.0650842386044304Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1460631.046386358943984Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1424891.0207824425047365No Hit
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1218780.8731265046957468Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC1183800.8480670475876081Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1179940.8453017673006609Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC968560.6938704338667459No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG955790.6847220843164048TruSeq Adapter, Index 5 (100% over 37bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA926530.6637604000687165No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG839580.6014699542267309Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC805460.5770265958353732No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT805250.5768761531254617Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTC741930.5315140941178189No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA717810.5142346742936822Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCCCCGAATCCGGAGTGAGATCGGAAGAGCACACGTCTGAACTCCAG716780.5134967886212585Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA683870.48992026679653455Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT669600.47969732646111035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT609910.43693577715336895No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC608230.4357322354740758Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC575970.41262136965622126Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC565160.4048771520650554Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG563690.403824053095674Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT556880.39894541093139657Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG536940.3846605174283581TruSeq Adapter, Index 5 (100% over 49bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT534730.3830772870050023Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA530970.3803836461037272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT527200.37768284126388496No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC515160.369057459228951No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT500870.3588201910163924No Hit
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA490640.3514914818621254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA479260.34333891977262804Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTC478590.34285893588862426No Hit
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC476700.3415049514994195Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT429480.3076768335850025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC415950.2979840247035526Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGCAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA409940.29367849762465287Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA402000.2879903304022795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA400730.28708051020424247Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT396630.28414329539168187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG395520.2833480982107204No Hit
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC384990.27580447089943677Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG384940.2757686512066007Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA384750.2756325363738235No Hit
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA379850.27212220647588525Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA374620.2683754666052287Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA357370.2560176725767727Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT357300.25596752500680214Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT351340.2516978176207385TruSeq Adapter, Index 5 (100% over 38bp)
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC345170.24727766752476324Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC343980.24642515883526395Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC319540.22891649297697608Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC292050.20922282585568588No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC291270.20866403864744265No Hit
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG289470.20737452970534292Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCCCGTCCTTTCCGCCCGGGAGATCGGAAGAGCACACGTCTGAACTC285540.20455910184842507Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC283550.20313347807354815TruSeq Adapter, Index 5 (100% over 35bp)
ACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCACACGTCTGAACTCCA271890.1947803257041686Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT271220.1943003418201648No Hit
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA256090.1834613027679596Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG253830.18184225265176768Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA250680.17958561200309311Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGAGATCGGAAGAGCACACGT250620.17954262837168977No Hit
AACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA248810.1782459554910228TruSeq Adapter, Index 5 (100% over 36bp)
AAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA248230.17783044705412399Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG242980.17406937930633304No Hit
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC241490.17300195245981712No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA240840.17253629645294777Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA240040.1719631813675701No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC237810.17036562306707984No Hit
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT233080.16697708012478435No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT230950.1654511612099663No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC230690.16526489880721856No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC227300.16283632363293066Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT225490.1615396507522637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC225180.16131756865667984TruSeq Adapter, Index 5 (100% over 35bp)
TAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC224250.1606513223699283TruSeq Adapter, Index 5 (100% over 35bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG221170.15844482929122428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT218320.15640310679956632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT213850.15320082626001857No Hit
TAAGGGCAGGGCGCCCTGGAATGGGTTCGAGATCGGAAGAGCACACGTCT210750.15098000530418013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG205180.1469896915222381Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG201130.14408829640251364No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC198000.1418459836309735No Hit
TTGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT197130.14122272097562527Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGTCTGAACT195860.14031290077758823Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG188370.13494711079073976Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA186930.13391550363705998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCA185140.13263315863352745Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC183740.1316302072341165No Hit
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG183350.1313508136299949No Hit
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA182820.13097112488593218Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT182810.13096396094736498No Hit
ATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGT178460.1278476476706239Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG178310.12774018859211558No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC175960.12605666302881866TruSeq Adapter, Index 5 (100% over 35bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG174790.12521848221645382Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACACGTCT173380.12420836687847567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCT172860.12383584207298018Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG172140.12332003849614029TruSeq Adapter, Index 5 (100% over 37bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC170990.12249618556090988Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA165940.11887839658446334Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTAGCGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT165640.11866347842744672Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG164280.11768918278230467Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC163360.11703010043412035No Hit
ATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA163230.11693696923274649Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCTG163200.11691547741704482Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC162140.1161560999289194Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGGGCAGGGCGCCCTGGAATGGGTTCGAGATCGGAAGAGCACACGTCT156160.11187206466572132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC153100.10967989946415173No Hit
TAATGCAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA150190.10759519334109044Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC150040.10748773426258212No Hit
AAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT145580.10429261766160161Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAA145350.10412784707455555Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGA143950.10312489567514461Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT143820.10303176447377074No Hit
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG143500.10280251843961968Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTC143260.10263058391400638No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA141310.10123361589339828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCGCACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGT141200.10115481256915888No Hit
ATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACTC140390.10057453354521398Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)