FastQCFastQC Report
Tue 4 Jul 2023
SRR6880604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14768347
Total Bases738.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA4307202.916507852910011Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA4111782.784184309862167Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG3013802.0407158634612257Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2671101.8086655195737207No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT2477201.677371204780061Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC1866341.2637433288911752Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT1852771.2545547582271732No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1538731.0419107839218567No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1484501.0051903574584211No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1207420.8175728807022208No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTC1183760.8015521303772182No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1076710.7290660220808733No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT1062540.7194711771060092Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1005390.6807735489963771No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC989500.6700140509970411Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA972880.658760252586156TruSeq Adapter, Index 6 (100% over 37bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG968130.6555439142918297No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT934550.6328060953605709Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA842400.5704091324506392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC822240.5567583156056666Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT796930.5396203109257929Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT791750.5361128093753484Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC722340.48911364284709724Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA702660.4757878454508145Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC677880.45900871641220237Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG663130.4490211396035047Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC630340.42681824851488115Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC609540.4127340724049889Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA571310.3868476275645473Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGAGATCGGAAGAGCACACGT525350.3557270153524968No Hit
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC523170.35425088535636384Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC514440.3483395941333177No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC468790.3174288903152127No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT463960.3141583821127713Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT457550.3098180182250593No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA454680.30787467277143477Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG442000.2992887423352119Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT430780.2916914127220873Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC427990.2898022371765777Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA419530.2840737693934196Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT408170.2763816424410938No Hit
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC402060.2722444157088129Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG382040.2586883962030415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG361680.24490215458778156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA356970.24171290124751268Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT356610.2414691366609953No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG356400.2413269406521935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT354810.24025031372840847Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT347420.23524636846628805Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT345820.23416297030398867Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA343100.23232119342807966No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA338520.2292199661884976Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG334650.2265994968834359Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA331210.22427019083449215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTC325190.22019390524884064No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT319340.21623273071793345No Hit
ATTAGCGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT316990.21464148966705618Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCCCGTCCTTTCCGCCCGGGAGATCGGAAGAGCACACGTCTGAACTC314660.21306379109320767Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG307720.20836455156423395TruSeq Adapter, Index 6 (100% over 49bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC306440.20749783303439442No Hit
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC286530.19401629715228116No Hit
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC269710.1826270739711086Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA261660.17717622696703972Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC260350.17628919472165708Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC255070.172713980786069No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA252910.1712513932669648No Hit
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG248640.16836007442132828Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT234310.1586568896302342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG231160.15652394949820722Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC230540.15610413271031617No Hit
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG226120.15311124528696407No Hit
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA225160.1524612063895844Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG221920.15026732511092813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG218260.14778905181466823Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT216600.14666502622128258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGTCGGGGCACGCCGGCCAGATCGGAAGAGCACACGTCTGAACTCC208310.14105166949286876Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTC205920.13943334348793401No Hit
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA204360.13837703027969211TruSeq Adapter, Index 6 (100% over 38bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG203870.1380452395924879No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA200550.13579718840571664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGAGATCGGAAGAGCACACGTCT195610.1324521965796172Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTCT194920.1319849811221256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT193730.1311792037389154No Hit
ATCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA191810.1298791259441561Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT188990.1279696366831034No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT188040.12732636902423813No Hit
ATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAACT187410.12689978099783272Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA184740.12509186031449557Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG184560.12496997802123691Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGA180940.12251878967903448Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG180340.1221125153681722No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGT179510.1215505025714794No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA179360.12144893399376382Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC178030.12054835927135243No Hit
AGGGCGCACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGT172850.11704085772090811No Hit
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT170510.11545638790854522No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACG170320.11532773437677216No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAA168220.11390577428875417Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTCTG167930.11370940837183742Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC167890.11368232341777992No Hit
ATGGGCGAGTGCCGTTCCGAAGGGACGGGCGAGATCGGAAGAGCACACGT166870.11299165708931407No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA166230.11255829782439429Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC166210.11254475534736555No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAGATCGGAAGAGCACAC165160.11183377530335657No Hit
TTTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT164630.11147489966209488Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGT162570.1100800245281344Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG162260.10987011613418887Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA160220.10848878347725714Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACACGTCT159730.10815699279005295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT158770.1075069538926733Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC157430.10659960793174754Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT154760.1047916872484104No Hit
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG154210.10441926913012No Hit
ACACATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGA148430.10050549326881336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT147950.10018047382012354No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)