FastQCFastQC Report
Tue 4 Jul 2023
SRR6880605.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880605.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11028969
Total Bases551.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1634951.4824141767013763No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1627361.4755323004353353Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1554241.4092341722966129Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1519531.377762508898157Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1411391.2797116394107193No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1370151.2423192049954985No Hit
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1006260.9123790265436416Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC972640.8818956694864224Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT921940.8359258240729482No Hit
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA910440.8254987388213713Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG859670.7794654241933221Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT856640.776718113905298Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG794540.720411853546782No Hit
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT736920.6681676229210546Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC727150.6593091339725409No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG676390.6132848863751453TruSeq Adapter, Index 7 (100% over 49bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC646960.5866006151617617Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA646190.5859024538014388Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC583980.5294964561057338No Hit
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACACG580670.5264952689594105No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC553630.5019780180722242No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC547780.4966738051399002Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA517830.46951804833253225Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA508530.46108570982473523TruSeq Adapter, Index 7 (100% over 37bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG505100.4579757183105692No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA458840.416031634507269Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTC444420.40295697630485683No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT443670.4022769490058409Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT431610.39134211003766534Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC418640.3795821712800172No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTC408860.37071461530085No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT401710.3642316883835651Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA397410.3603328652025407Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAA392870.3562164332858312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC381060.34550827008399426Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG378700.3433684508497576No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA374390.3394605606380796No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT373610.3387533322471031No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC362860.32900627429454193Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC362400.32858919088447885No Hit
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC356720.32343911747326515Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA356700.3234209834119581Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTCGTGTGGAACCTGGCGCTAAACCAGATCGGAAGAGCACACGTCTG333070.3019955899776307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCGTCCGAAAGCCGCCGTGGCGCAGATCGGAAGAGCACACGTCT324500.29422514470754246Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC322690.2925840121592508Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA321700.2916863761245498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG310130.28119582165839796Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTAGAGGAAGTAAAAGTCGTAACAGATCGGAAGAGCACACGTCTGAAC301980.27380619167575865Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG300150.27214692506615984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC299890.27191118226916766No Hit
TCCTACTTGGATAACTGTGGTAATTCAGATCGGAAGAGCACACGTCTGAA295460.2678944876896472Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA295010.2674864713102376Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT291090.2639321952940479Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT278040.25209972029117134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTG274680.24905319799158016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT272510.24708565233976088Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA261900.23746553281634936Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC255680.23182583974984428Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC253780.23010310392567065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA251720.22823529561104036Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT250300.22694777725823695Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC240020.21762686974639242Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT238230.21600387125940784Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC236790.21469821884529733No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGAGATCGGAAGAGCACAC234970.2130480192663521No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT232190.21052738474466653No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC230650.20913106202402054No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA227970.20670109780887044Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC219860.19934773594884525Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCTTGGTGGTAGTAGCAAATAGATCGGAAGAGCACACGTCTGAACTC215360.1952675721547499Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTC211760.19200344111947365No Hit
ATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTGAACT208110.18869397493092963Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAGATCGGAAGAGCAC204580.18549331311022815No Hit
AAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGAA200800.18206597552318807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG194820.176643891192368Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA193620.1755558475139426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT190330.17257279442892623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA190080.1723461186625876Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA185610.16829315596045288Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCACACGTCT185320.16803021207150007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAACGATCTCAACCTATTCTCAAACAGATCGGAAGAGCACACGTCTGAA183400.16628934218601937Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGATCGGAAGAGCACACGT182060.1650743600784443No Hit
TCCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC181480.16454847230053868Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAA180750.16388657906282988Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC174290.1580292772606397No Hit
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG172640.15653321720280472Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA172090.15603453051685973Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCACACGTCTGAACTCCA171550.1555449108615683Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTG169920.15406698486504042Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACAC166900.15132874160766976No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG166250.15073938461518932No Hit
AGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAAC165350.14992335185637026Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA165130.14972387718199226No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG159240.14438339612705414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT158210.14344949196973897Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGTCTGAACT156870.1422345098621639Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAAC153580.13925145677714754Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGAATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGAAC151240.13712977160421794Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC150820.13674895631676903No Hit
AAAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGA148860.1349718183086742Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC147580.1338112383850204No Hit
TACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACAC147430.1336752329252172No Hit
AAAACGATCTCAACCTATTCTCAAACTTTAGATCGGAAGAGCACACGTCT142940.1296041361617754Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC140400.12730111037577493Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTC140100.12702909945616858No Hit
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG138740.12579598328728642Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGATCCTTCTGACCTTTTGGGTTTTAGATCGGAAGAGCACACGTCTGAA136740.12398257715657737Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG136410.12368336514501038No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT136210.12350202453193948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC135530.12288546644749841No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACAC133280.12084538455045073No Hit
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT132450.12009282100620647No Hit
TAGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTG131250.11900477732778106Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC130860.11865116313229279No Hit
ATTAGCGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT129090.11704629870661527Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA127870.11594012096688276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACGAGAACTTTGAAGGCCGAAAGATCGGAAGAGCACACGTCTGAACT127120.11526009366786685Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC125570.11385470391656737No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT122320.11090791895416517No Hit
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC121050.10975640606116492Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA119640.10847795473901505TruSeq Adapter, Index 7 (100% over 35bp)
TCGTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC118020.1070090957731407Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT118010.10700002874248717No Hit
ACACCGCCCGTCGCTACTACCGATTAGATCGGAAGAGCACACGTCTGAAC117440.10648320799523509Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACG115840.10503248309066786No Hit
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT115520.10474233810975442TruSeq Adapter, Index 7 (100% over 38bp)
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA115310.10455193046602995Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG111100.10073471056088742No Hit
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA111040.10068030837696615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)